PMID- 11135672
OWN - NLM
STAT- MEDLINE
DCOM- 20010118
LR  - 20131121
IS  - 1072-8368 (Print)
IS  - 1072-8368 (Linking)
VI  - 8
IP  - 1
DP  - 2001 Jan
TI  - Crystal structure of a DNA-dependent RNA polymerase (DNA primase).
PG  - 57-61
AB  - Primases are essential components of the DNA replication apparatus in every 
      organism. They catalyze the synthesis of oligoribonucleotides on single-stranded 
      DNA, which subsequently serve as primers for the replicative DNA polymerases. In 
      contrast to bacterial primases, the archaeal enzymes are closely related to their 
      eukaryotic counterparts. We have solved the crystal structure of the catalytic 
      primase subunit from the hyperthermophilic archaeon Pyrococcus furiosus at 2.3 A 
      resolution by multiwavelength anomalous dispersion methods. The structure shows a 
      two-domain arrangement with a novel zinc knuckle motif located in the primase 
      (prim) domain. In this first structure of a complete protein of the 
      archaeal/eukaryotic primase family, the arrangement of the catalytically active 
      residues resembles the active sites of various DNA polymerases that are unrelated 
      in fold.
FAU - Augustin, M A
AU  - Augustin MA
AD  - Max-Planck-Institut fur Biochemie, Abteilung fur Strukturforschung, Am 
      Klopferspitz 18A, D-82152 Martinsried, Germany. augustin@biochem.mpg.de
FAU - Huber, R
AU  - Huber R
FAU - Kaiser, J T
AU  - Kaiser JT
LA  - eng
SI  - PDB/1G71
PT  - Journal Article
PL  - United States
TA  - Nat Struct Biol
JT  - Nature structural biology
JID - 9421566
RN  - 0 (Protein Subunits)
RN  - EC 2.7.7.- (DNA Primase)
RN  - H6241UJ22B (Selenium)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
CIN - Nat Struct Biol. 2001 Jan;8(1):2-4. PMID: 11135655
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Catalytic Domain
MH  - Crystallography, X-Ray
MH  - DNA Primase/*chemistry/metabolism
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Protein Structure, Secondary
MH  - Protein Structure, Tertiary
MH  - Protein Subunits
MH  - Pyrococcus furiosus/*enzymology
MH  - Selenium/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Zinc/metabolism
EDAT- 2001/01/03 11:00
MHDA- 2001/02/28 10:01
CRDT- 2001/01/03 11:00
PHST- 2001/01/03 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2001/01/03 11:00 [entrez]
AID - 10.1038/83060 [doi]
PST - ppublish
SO  - Nat Struct Biol. 2001 Jan;8(1):57-61. doi: 10.1038/83060.