PMID- 11139213
OWN - NLM
STAT- MEDLINE
DCOM- 20010322
LR  - 20181113
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 8
IP  - 1
DP  - 2001 Jan
TI  - Comparison of specific serological assays for diagnosing human herpesvirus 6 
      infection after liver transplantation.
PG  - 170-3
AB  - Cross-reactivity between human herpesvirus 6 (HHV-6) and human herpesvirus 7 
      (HHV-7) antibodies and the reliability of specific serological assays were 
      analyzed for 12 patients with concurrent HHV-6 and HHV-7 antibody responses after 
      transplantation with a liver from a living relative by using an 
      immunofluorescence assay (IFA). A neutralizing antibody titer assay (NT) and an 
      immunoblot assay (IB) designed to detect immunoglobulin M (IgM) antibody to the 
      HHV-6 immunodominant 101-kDa protein were compared in the diagnosis of an active 
      HHV-6 infection. A total of 9 of 12 patients demonstrated concurrent HHV-6 and 
      HHV-7 antibody responses, including increased IgG titers and/or the presence of 
      IgM by IFA, and were thus analyzed for cross-reactive antibody to heterologous 
      virus. The average percentages of residual antibody to HHV-6 and HHV-7 after 
      absorption with HHV-6 antigen were 32.6% (range, 6 to 50%) and 55.6% (range, 35 
      to 100%), respectively. All 12 patients were subsequently analyzed for HHV-6 
      antibody by using IB and NT. IB detected IgM antibody to the 101-kDa protein in 
      75% (9 of 12) of the recipients. A significant rise in the NT antibody titer was 
      detected in the same nine samples. However, HHV-6 DNA was detected by PCR in only 
      five of nine plasma samples collected from recipients with a specific serologic 
      response against HHV-6.
FAU - Yoshikawa, T
AU  - Yoshikawa T
AD  - Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, 
      Aichi, Japan. tetsushi@med.nagoya-u.ac.jp
FAU - Black, J B
AU  - Black JB
FAU - Ihira, M
AU  - Ihira M
FAU - Suzuki, K
AU  - Suzuki K
FAU - Suga, S
AU  - Suga S
FAU - Iida, K
AU  - Iida K
FAU - Saito, Y
AU  - Saito Y
FAU - Asonuma, K
AU  - Asonuma K
FAU - Tanaka, K
AU  - Tanaka K
FAU - Asano, Y
AU  - Asano Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (101K protein, Human herpesvirus 6)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibodies, Viral/*blood/immunology
MH  - Child
MH  - Child, Preschool
MH  - Herpesviridae Infections/blood/*diagnosis/etiology/immunology
MH  - Herpesvirus 6, Human/immunology/*isolation & purification
MH  - Herpesvirus 7, Human/immunology
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin G/blood/immunology
MH  - Immunoglobulin M/blood/immunology
MH  - Infant
MH  - Liver Transplantation/*adverse effects
MH  - Middle Aged
MH  - Neutralization Tests
MH  - Viral Proteins/*immunology
PMC - PMC96028
EDAT- 2001/01/04 11:00
MHDA- 2001/03/27 10:01
PMCR- 2001/01/01
CRDT- 2001/01/04 11:00
PHST- 2001/01/04 11:00 [pubmed]
PHST- 2001/03/27 10:01 [medline]
PHST- 2001/01/04 11:00 [entrez]
PHST- 2001/01/01 00:00 [pmc-release]
AID - 0051 [pii]
AID - 10.1128/CDLI.8.1.170-173.2001 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2001 Jan;8(1):170-3. doi: 10.1128/CDLI.8.1.170-173.2001.