PMID- 11139516
OWN - NLM
STAT- MEDLINE
DCOM- 20010315
LR  - 20210915
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 157
IP  - 1
DP  - 2001 Jan
TI  - Development and applications of a complete set of rice telotrisomics.
PG  - 361-8
AB  - We previously isolated a complete set of primary trisomics along with many other 
      aneuploids from triploid plants derived from an indica rice variety "Zhongxian 
      3037." About 30,000 progeny from these trisomic and aneuploid plants were grown 
      each year from 1994 to 1999. The variants that differed morphologically from both 
      the diploids and the original primary trisomics were collected for cytological 
      identification. From these variants, a complete set of telotrisomics covering all 
      24 rice chromosome arms was obtained. The identities of the extra chromosomes 
      were further confirmed by dosage analysis of the RFLP markers on extra chromosome 
      arms. The telocentric nature of the extra chromosomes in these stocks was 
      verified by fluorescence in situ hybridization (FISH) using a rice centromeric 
      BAC clone as a marker probe. In general, the shorter the extra chromosome arm of 
      a telotrisomic, the stronger the resemblance it bears to the diploid; the longer 
      the extra chromosome arm, the stronger the resemblance to the corresponding 
      primary trisomic. We demonstrated that DNA clones can be rapidly assigned to 
      specific chromosome arms by dosage analysis with the telotrisomics. We also 
      showed that telotrisomics are valuable tools for chromosome microdissection and 
      for developing chromosome-specific DNA markers.
FAU - Cheng, Z
AU  - Cheng Z
AD  - Yangzhou University, Yangzhou 225009, People's Republic of China.
FAU - Yan, H
AU  - Yan H
FAU - Yu, H
AU  - Yu H
FAU - Tang, S
AU  - Tang S
FAU - Jiang, J
AU  - Jiang J
FAU - Gu, M
AU  - Gu M
FAU - Zhu, L
AU  - Zhu L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Chromosomes/genetics
MH  - DNA, Plant/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Oryza/*genetics
MH  - Phenotype
MH  - Ploidies
MH  - Polymorphism, Restriction Fragment Length
MH  - Trisomy
PMC - PMC1461484
EDAT- 2001/01/05 11:00
MHDA- 2001/03/17 10:01
PMCR- 2001/04/01
CRDT- 2001/01/05 11:00
PHST- 2001/01/05 11:00 [pubmed]
PHST- 2001/03/17 10:01 [medline]
PHST- 2001/01/05 11:00 [entrez]
PHST- 2001/04/01 00:00 [pmc-release]
AID - 10.1093/genetics/157.1.361 [doi]
PST - ppublish
SO  - Genetics. 2001 Jan;157(1):361-8. doi: 10.1093/genetics/157.1.361.