PMID- 11140264 OWN - NLM STAT- MEDLINE DCOM- 20010125 LR - 20201208 IS - 0031-8655 (Print) IS - 0031-8655 (Linking) VI - 72 IP - 6 DP - 2000 Dec TI - The effect of a vitamin A acetate diet on ultraviolet radiation-induced immune suppression as measured by contact hypersensitivity in mice. PG - 766-71 AB - The adverse health effects caused by increased exposure to ultraviolet radiation (UVR) due to deterioration of stratospheric ozone are of major concern. These health effects include sunburn, skin cancer, cataracts and immune suppression. Immune suppression has been associated with the release of cytokines, a defect in antigen presentation, induction of suppressor T cells and suppression of contact hypersensitivity (CH). CH is typically assessed by the mouse ear swelling test (MEST). Previous studies have demonstrated enhanced CH responses with vitamin A acetate (VAA) dietary supplementation assessed by MEST and the local lymph node assay (LLNA). To determine the effect that VAA has on UVR-induced immune suppression, we examined both the induction and elicitation phases of CH using murine models. The MEST was used to evaluate the interaction of UVR and VAA on CH elicitation. However, a positive MEST response requires that the induction phase as well as the elicitation phase of CH be functional. The LLNA was used to evaluate the interaction of UVR and VAA only on CH induction. We tested the hypothesis that mice maintained on a VAA-enriched diet are more resistant to UVR-induced immune suppression (CH) than those maintained on a control diet. Mice were maintained on a VAA-enriched or the control diet for 3 weeks and then exposed to UVR 3 days prior to sensitization with 2,4-dinitrofluorobenzene (DNFB). VAA enhanced the MEST response in both UVR-exposed and non-UVR-exposed mice. The VAA-enriched diet did not significantly alter the LLNA response in either UVR- or non-UVR-exposed mice. However, there was significant suppression in CH by UVR as measured by the LLNA. These results indicate that (1) the VAA-enriched diet does not restore the number of proliferating cells in the CH induction phase of UVR-induced immunosuppression; (2) the immunosuppressive effects of UVR affect the induction phase of CH; and (3) the LLNA should be examined as an alternative to the MEST for measurement of UVR-induced immunosuppression. The data indicate that the VAA-enriched diet enhanced the elicitation response (MEST) but not the earlier induction phase (LLNA). Further studies are necessary to define mechanisms of action, but modulation of cytokines and effects of specific lymphocyte subsets, as well as systemic effects and local modulation at the site of elicitation are possible. Additionally, future studies to evaluate the effect of the VAA-enriched diet when multiple doses of both UVR and DNFB are used would be of interest for both the LLNA and MEST end-points. FAU - Sailstad, D M AU - Sailstad DM AD - United States Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Research Triangle Park, NC, USA. FAU - Boykin, E H AU - Boykin EH FAU - Slade, R AU - Slade R FAU - Doerfler, D L AU - Doerfler DL FAU - Selgrade, M K AU - Selgrade MK LA - eng PT - Journal Article PL - United States TA - Photochem Photobiol JT - Photochemistry and photobiology JID - 0376425 RN - 0 (Diterpenes) RN - 0 (Retinyl Esters) RN - 11103-57-4 (Vitamin A) RN - 3LE3D9D6OY (retinol acetate) SB - IM MH - Animals MH - Dermatitis, Contact/*immunology MH - Diterpenes MH - Female MH - Immune System/*radiation effects MH - Mice MH - Mice, Inbred BALB C MH - Retinyl Esters MH - *Ultraviolet Rays MH - Vitamin A/*administration & dosage/*analogs & derivatives EDAT- 2001/01/05 11:00 MHDA- 2001/02/28 10:01 CRDT- 2001/01/05 11:00 PHST- 2001/01/05 11:00 [pubmed] PHST- 2001/02/28 10:01 [medline] PHST- 2001/01/05 11:00 [entrez] AID - 10.1562/0031-8655(2000)072<0766:teoava>2.0.co;2 [doi] PST - ppublish SO - Photochem Photobiol. 2000 Dec;72(6):766-71. doi: 10.1562/0031-8655(2000)072<0766:teoava>2.0.co;2.