PMID- 11140760
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20010125
LR  - 20190717
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 72
IP  - 24
DP  - 2000 Dec 15
TI  - Dynamic coating using polyelectrolyte multilayers for chemical control of 
      electroosmotic flow in capillary electrophoresis microchips.
PG  - 5939-44
AB  - Poly(dimethylsiloxane) (PDMS) capillary electrophoresis (CE) microchips were 
      modified by a dynamic coating method that provided stable electroosmotic flow 
      (EOF) with respect to pH. The separation channel was coated with a polymer 
      bilayer consisting of a cationic layer of Polybrene (PB) and an anionic layer of 
      dextran sulfate (DS). According to the difference in charge, PB- and PB/ 
      DS-coated channels supported EOF in different directions; however, both methods 
      of channel coating exhibited a pH-independent EOF in the pH range of 5-10 due to 
      chemical control of the effective zeta-potential. The endurance of the PB-coated 
      layer was determined to be 50 runs at pH 3.0, while PB/DS-coated chips had a 
      stable EOF for more than 100 runs. The effect of substrate composition and 
      chip-sealing methodology was also evaluated. All tested chips showed the same EOF 
      on the PB/DS-coated channels, as compared to uncoated chips, which varied 
      significantly. No significant variation for separation and electrochemical 
      detection of dopamine and hydroquinone between coated and uncoated channels was 
      observed.
FAU - Liu, Y
AU  - Liu Y
AD  - Department of Chemistry, Mississippi State University, 39762, USA.
FAU - Fanguy, J C
AU  - Fanguy JC
FAU - Bledsoe, J M
AU  - Bledsoe JM
FAU - Henry, C S
AU  - Henry CS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
EDAT- 2001/01/05 11:00
MHDA- 2001/01/05 11:01
CRDT- 2001/01/05 11:00
PHST- 2001/01/05 11:00 [pubmed]
PHST- 2001/01/05 11:01 [medline]
PHST- 2001/01/05 11:00 [entrez]
AID - 10.1021/ac000932l [doi]
PST - ppublish
SO  - Anal Chem. 2000 Dec 15;72(24):5939-44. doi: 10.1021/ac000932l.