PMID- 11144223
OWN - NLM
STAT- MEDLINE
DCOM- 20010308
LR  - 20091119
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 58
IP  - 1
DP  - 2001 Jan
TI  - Colocalization of CaM KII and MAP kinase on architectural elements of the mouse 
      egg: potentiation of MAP kinase activity by CaM KII.
PG  - 69-77
AB  - The conversion of the egg to a zygote requires the initiation of several 
      signaling pathways that act in an orchestrated fashion to rapidly remodel the 
      egg. Architectural elements within the egg can serve to localize components of 
      these signaling pathways and colocalization of such components provides the 
      opportunity for interaction between different signaling pathways. This study 
      examines the localization as well as the state of activation of two different 
      kinases, MAP kinase and calcium/calmodulin-dependent protein kinase II (CaM KII). 
      The meiotic spindle serves as a site for enrichment of these kinases. However, 
      activated MAP kinase and activated CaM KII exhibit a developmental stage-specific 
      pattern of localization that represents a subset of the area occupied by the 
      distribution of the total mass of MAP kinase and CaM KII. Suppression of CaM KII 
      activity results in reduction in the amount of MAP kinase as well as a decreased 
      level of activity of MAP kinase. Since CaM KII becomes active as a result of 
      fertilization, the former kinase could serve to potentiate MAP kinase activity 
      and the colocalization of these two kinases may facilitate such an interaction.
FAU - Hatch, K R
AU  - Hatch KR
AD  - Department of Biology, Arizona State University, Tempe 85287-1501, USA.
FAU - Capco, D G
AU  - Capco DG
LA  - eng
GR  - HD 32621/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - Enzyme Activation
MH  - Female
MH  - Humans
MH  - MAP Kinase Signaling System
MH  - Mice
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Ovum/cytology/*enzymology
MH  - Signal Transduction
EDAT- 2001/01/06 11:00
MHDA- 2001/03/10 10:01
CRDT- 2001/01/06 11:00
PHST- 2001/01/06 11:00 [pubmed]
PHST- 2001/03/10 10:01 [medline]
PHST- 2001/01/06 11:00 [entrez]
AID - 10.1002/1098-2795(200101)58:1<69::AID-MRD10>3.0.CO;2-O [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2001 Jan;58(1):69-77. doi: 
      10.1002/1098-2795(200101)58:1<69::AID-MRD10>3.0.CO;2-O.