PMID- 11144959
OWN - NLM
STAT- MEDLINE
DCOM- 20010329
LR  - 20190607
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 9
IP  - 5
DP  - 2000 Sep-Oct
TI  - Neurotrophic factors NGF and FGF-2 alter levels of huntingtin (IT15) in striatal 
      neuronal cell cultures.
PG  - 623-7
AB  - A mutation of the human IT15 gene is responsible for Huntington's disease (HD) 
      and the causative factor in the major neuronal loss observed in the striatum. The 
      growth factors basic fibroblast growth factor (FGF-2), nerve growth factor (NGF), 
      and brain-derived neurotrophic factor (BDNF) improve survival and promote 
      differentiation of striatal neurons, as well as exert a neuroprotective effect 
      when such neurons are challenged with metabolic toxins or excitatory amino acids. 
      Using Western blotting and striatal cell cultures, we found that FGF-2 increased 
      the level of huntingtin in a dose-dependent fashion, whereas NGF decreased 
      huntingtin expression. The neurotrophic factor-specific, dose-dependent effect on 
      striatal levels of huntingtin may be relevant to understanding the normal role of 
      IT15 and developing new therapies against the disease provoking mutated IT15.
FAU - Haque, N S
AU  - Haque NS
AD  - Department of Neurology, Massachusetts General Hospital, Belmont 02178, USA.
FAU - Isacson, O
AU  - Isacson O
LA  - eng
GR  - NS30064/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Htt protein, rat)
RN  - 0 (Huntingtin Protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proteins)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cells, Cultured
MH  - Corpus Striatum/drug effects/*metabolism
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Gene Expression
MH  - Huntingtin Protein
MH  - Nerve Growth Factor/*pharmacology
MH  - Nerve Tissue Proteins
MH  - Nuclear Proteins
MH  - Proteins/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2001/01/06 11:00
MHDA- 2001/04/03 10:01
CRDT- 2001/01/06 11:00
PHST- 2001/01/06 11:00 [pubmed]
PHST- 2001/04/03 10:01 [medline]
PHST- 2001/01/06 11:00 [entrez]
AID - 10.1177/096368970000900507 [doi]
PST - ppublish
SO  - Cell Transplant. 2000 Sep-Oct;9(5):623-7. doi: 10.1177/096368970000900507.