PMID- 11145421
OWN - NLM
STAT- MEDLINE
DCOM- 20010301
LR  - 20211203
IS  - 0016-6707 (Print)
IS  - 0016-6707 (Linking)
VI  - 108
IP  - 1
DP  - 2000
TI  - Cyclic core dendrimer as a new kind of vector for gene transfer into mammalian 
      cells.
PG  - 53-6
AB  - Cyclic core dendritic polymer is a new type of synthetic polymers. The ability of 
      generation 4 of the dendrimer with a core of 1,4,7,10-tetraazacyclododecane to 
      function as an effective gene delivery vector was investigated. Results from 
      fluorescence in situ hybridization (FISH) show that the pCH 110 plasmid DNA was 
      transferred into human small intestine cancer metastatic ascites (HICMA) cells 
      induced by this kind of dendrimer as a vector. The transferred LacZ, GFP and 
      luciferase genes were highly expressed in the transfected HICMA, COS-7 and 293 
      cells. These studies demonstrate that the dendrimer can transfect mammalian cells 
      in vitro which offers an alternatively efficient method for mammalian gene 
      transfer.
FAU - Cheng, H
AU  - Cheng H
AD  - Center for Developmental biology, College of Life Sciences, Wuhan University, PR 
      China.
FAU - Zhou, R
AU  - Zhou R
FAU - Liu, L
AU  - Liu L
FAU - Du, B
AU  - Du B
FAU - Zhuo, R
AU  - Zhuo R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Genetica
JT  - Genetica
JID - 0370740
RN  - 0 (Cyclams)
RN  - 0 (Heterocyclic Compounds)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Polymers)
RN  - 9007-49-2 (DNA)
RN  - 964584YO2O (cyclen)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Cell Line
MH  - Cyclams
MH  - DNA/analysis/metabolism
MH  - Gene Expression
MH  - *Gene Transfer Techniques
MH  - Genes, Reporter/genetics
MH  - Genetic Vectors/chemistry/*genetics
MH  - Heterocyclic Compounds/chemistry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Luciferases/biosynthesis/genetics
MH  - Macromolecular Substances
MH  - Polymers/chemistry
MH  - Transfection
MH  - beta-Galactosidase/biosynthesis/genetics
EDAT- 2001/01/06 11:00
MHDA- 2001/03/07 10:01
CRDT- 2001/01/06 11:00
PHST- 2001/01/06 11:00 [pubmed]
PHST- 2001/03/07 10:01 [medline]
PHST- 2001/01/06 11:00 [entrez]
AID - 10.1023/a:1004047902652 [doi]
PST - ppublish
SO  - Genetica. 2000;108(1):53-6. doi: 10.1023/a:1004047902652.