PMID- 11149241
OWN - NLM
STAT- MEDLINE
DCOM- 20010308
LR  - 20190921
IS  - 0161-6412 (Print)
IS  - 0161-6412 (Linking)
VI  - 22
IP  - 8
DP  - 2000 Dec
TI  - Elevated transferrin concentration in cerebral spinal fluid after subarachnoid 
      hemorrhage.
PG  - 797-801
AB  - Calcium-elevating protein cross-reacted with anti-human transferrin (TF) was 
      purified in cerebrospinal fluid (CSF) after subarachnoid hemorrhage (SAH). The 
      concentration of TF in CSF after SAH was measured, and the effects of TF on 
      cultured smooth muscle cells (SMCs) were evaluated in order to understand the 
      relationship between TF and cerebral vasospasm. Cisternal CSF samples were 
      collected from 12 patients (seven men and 13 women) with SAH due to the rupture 
      of a cerebral aneurysm, and eight control subjects. The patients were divided 
      into two groups: (1) those presenting no clinical and radiological evidence of 
      vasospasm (the non-vasospasm group; three men and 10 women, mean age 54.7 years), 
      and (2) those presenting evidence of vasospasm (the vasospasm group; four men and 
      three women, mean age 58.3 years). The concentration of TF in CSF was measured 
      using Speriol micro-transferrin measure assay method. Nitrite accumulation in the 
      culture media of SMCs incubated with TF was determined by diazotization method. 
      The mRNA levels of inducible isoform of NOS (iNOS) in SMC incubated with TF were 
      measured by the reverse transcription-coupled polymerase chain reaction method. 
      Levels of TF were markedly different in the SAH and the control subjects. In the 
      control group, all subjects had no detectable quantity of TF. In contrast, all 
      patients after SAH had quantifiable TF in their CSF. Moreover, there was a 
      significant difference between the vasospasm group and the non-vasospasm group in 
      TF levels (p < 0.05). In the vasospasm group, the average value was 10.43 +/- 2.8 
      mg dl-1. In the non-vasospasm group, the average was 3.69 +/- 0.31 mg dl-1. The 
      nitrite content in the culture medium incubated with TF was three times the 
      content of control. TF also induced iNOS mRNA in SMC. This study demonstrated 
      that an elevation of TF concentration in CSF after SAH was detected and iNOS mRNA 
      in SMCs was also induced by TF. This may be involved in some roles of the 
      development of the pathological series of events after SAH, including vasospasm.
FAU - Takenaka, K V
AU  - Takenaka KV
AD  - Department of Neurosurgery, Gifu University School of Medicine, Gifu, Japan. 
      kat927@oregano.ocn.ne.jp
FAU - Sakai, N
AU  - Sakai N
FAU - Murase, S
AU  - Murase S
FAU - Kuroda, T
AU  - Kuroda T
FAU - Okumura, A
AU  - Okumura A
FAU - Sawada, M
AU  - Sawada M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neurol Res
JT  - Neurological research
JID - 7905298
RN  - 0 (Nitrites)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transferrin)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Aorta, Thoracic/cytology/metabolism
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Muscle, Smooth, Vascular/cytology/drug effects/*metabolism
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase/genetics/metabolism
MH  - Nitric Oxide Synthase Type II
MH  - Nitrites/analysis
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Subarachnoid Hemorrhage/*cerebrospinal fluid
MH  - Transferrin/*cerebrospinal fluid/pharmacology
MH  - Vasospasm, Intracranial/cerebrospinal fluid
EDAT- 2001/01/10 11:00
MHDA- 2001/03/10 10:01
CRDT- 2001/01/10 11:00
PHST- 2001/01/10 11:00 [pubmed]
PHST- 2001/03/10 10:01 [medline]
PHST- 2001/01/10 11:00 [entrez]
AID - 10.1080/01616412.2000.11740755 [doi]
PST - ppublish
SO  - Neurol Res. 2000 Dec;22(8):797-801. doi: 10.1080/01616412.2000.11740755.