PMID- 11151448
OWN - NLM
STAT- MEDLINE
DCOM- 20010315
LR  - 20190720
IS  - 0302-766X (Print)
IS  - 0302-766X (Linking)
VI  - 302
IP  - 3
DP  - 2000 Dec
TI  - Induction of the synthesis of the C-X-C chemokine interferon-gamma-inducible 
      protein-10 in experimental canine endotoxemia.
PG  - 365-76
AB  - Endotoxemia is associated with a systemic inflammatory response leading to 
      organ-specific leukocyte recruitment and tissue injury. Chemokine expression has 
      been demonstrated in various models of sepsis and may mediate tissue infiltration 
      with inflammatory cells. In this study we examined expression of the C-X-C 
      chemokine interferon-gamma-inducible protein-10 (IP-10), a potent T-lymphocyte 
      chemoattractant, in a canine model of endotoxemia and investigated mechanisms of 
      cytokine-mediated IP-10 induction in endothelial cells. Control canine tissues 
      showed negligible expression of IP-10 message, with the exception of the spleen. 
      Endotoxemic dogs demonstrated a robust induction of IP-10 mRNA in the heart, 
      lung, kidney, liver, and spleen. Immunohistochemical studies indicated that IP-10 
      was predominantly localized in cardiac venular endothelial cells, bronchial 
      epithelial cells, renal mesangial cells, and in the splenic red pulp of 
      endotoxemic dogs. In addition, IP-10 expression was associated with T-lymphocyte 
      infiltration in canine tissues. Tumor necrosis factor-alpha (TNF-alpha) and 
      interleukin-1 beta (IL-1 beta) induced a marked upregulation of IP-10 message in 
      canine venular endothelial cells. IP-10 expression in TNF-alpha-stimulated 
      endothelial cells peaked at 6 h of stimulation and returned to baseline levels 
      after 24 h. In addition, macrophage colony-stimulating factor (M-CSF) induced a 
      dose-dependent induction of IP-10 mRNA in canine endothelial cells. 
      M-CSF-mediated IP-10 expression peaked after 6 h of incubation and returned to 
      baseline levels after 24 h. Canine endotoxemia is associated with a robust early 
      expression of IP-10 in multiple tissues. IP-10 induction may be important in 
      regulating lymphocyte recruitment and function. TNF-alpha, IL-1 beta, and M-CSF 
      are potent inducers of IP-10 in canine endothelial cells and may indirectly 
      mediate lymphocyte chemotaxis and activation in inflammatory processes.
FAU - Frangogiannis, N G
AU  - Frangogiannis NG
AD  - Section of Cardiovascular Sciences, Department of Medicine, One Baylor Plaza M/S 
      F-602, Baylor College of Medicine, Houston, TX 77030, USA. ngf@bcm.tmc.edu
FAU - Mendoza, L H
AU  - Mendoza LH
FAU - Smith, C W
AU  - Smith CW
FAU - Michael, L H
AU  - Michael LH
FAU - Entman, M L
AU  - Entman ML
LA  - eng
GR  - HL-42550/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Endotoxins)
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Chemokine CXCL10
MH  - Chemokines, CXC/biosynthesis/*genetics
MH  - Dogs
MH  - Endothelium/cytology/drug effects/immunology
MH  - Endotoxemia/*immunology
MH  - Endotoxins/pharmacology
MH  - Female
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Macrophage Colony-Stimulating Factor/pharmacology
MH  - Male
MH  - Molecular Sequence Data
MH  - RNA, Messenger/biosynthesis
MH  - Sequence Homology, Nucleic Acid
MH  - T-Lymphocytes/immunology
MH  - Transcriptional Activation
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2001/01/11 11:00
MHDA- 2001/03/17 10:01
CRDT- 2001/01/11 11:00
PHST- 2001/01/11 11:00 [pubmed]
PHST- 2001/03/17 10:01 [medline]
PHST- 2001/01/11 11:00 [entrez]
AID - 10.1007/s004410000274 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2000 Dec;302(3):365-76. doi: 10.1007/s004410000274.