PMID- 11154209
OWN - NLM
STAT- MEDLINE
DCOM- 20010315
LR  - 20220425
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 97
IP  - 2
DP  - 2001 Jan 15
TI  - Signal transduction involved in MCP-1-mediated monocytic transendothelial 
      migration.
PG  - 359-66
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a major chemoattractant for 
      monocytes and T lymphocytes. The MonoMac6 cell line was used to examine MCP-1 
      receptor-mediated signal transduction events in relation to MCP-1-mediated 
      monocytic transendothelial migration. MCP-1 stimulates, with distinct time 
      courses, extracellular signal-related kinases (ERK1 and ERK2) and 
      stress-activated protein kinases (SAPK1/JNK1 and SAPK2/p38). SAPK1/JNK1 
      activation was blocked by piceatannol, indicating that it is regulated by Syk 
      kinase, whereas SAPK2/p38 activation was inhibited by PP2, revealing an upstream 
      regulation by Src-like kinases. In contrast, ERK activation was insensitive to 
      PP2 and piceatannol. Pertussis toxin, a blocker of Go/Gi proteins, abrogated 
      MCP-1-induced ERK activation, but was without any effect on SAPK1/JNK1 and 
      SAPK2/p38 activation. These results underscore the major implication of Go/Gi 
      proteins and nonreceptor tyrosine kinases in the early MCP-1 signaling. 
      Furthermore, MCP-1-mediated chemotaxis and transendothelial migration were 
      significantly diminished by a high concentration of SB202190, a broad SAPK 
      inhibitor, or by SB203580, a specific inhibitor of SAPK2/p38, and abolished by 
      pertussis toxin treatment. Altogether, these data suggest that coordinated action 
      of distinct signal pathways is required to produce a full response to MCP-1 in 
      terms of monocytic locomotion.
FAU - Cambien, B
AU  - Cambien B
AD  - INSERM U364, Faculte de Medecine, Nice, France.
FAU - Pomeranz, M
AU  - Pomeranz M
FAU - Millet, M A
AU  - Millet MA
FAU - Rossi, B
AU  - Rossi B
FAU - Schmid-Alliana, A
AU  - Schmid-Alliana A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Chemokine CCL2)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Enzyme Precursors)
RN  - 0 (Fibronectins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 9012-63-9 (Cholera Toxin)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (SYK protein, human)
RN  - EC 2.7.10.2 (Syk Kinase)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 8)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - Cell Line
MH  - Chemokine CCL2/*pharmacology
MH  - Chemotaxis/drug effects
MH  - Cholera Toxin/pharmacology
MH  - Endothelium, Vascular/cytology/physiology
MH  - Enzyme Activation/drug effects
MH  - Enzyme Inhibitors/pharmacology
MH  - Enzyme Precursors/drug effects/metabolism
MH  - Fibronectins/physiology
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Kinetics
MH  - Mitogen-Activated Protein Kinase 8
MH  - Mitogen-Activated Protein Kinase Kinases/drug effects/metabolism
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
MH  - Monocytes/*cytology/drug effects
MH  - Pertussis Toxin
MH  - Protein-Tyrosine Kinases/drug effects/metabolism
MH  - Signal Transduction/*drug effects
MH  - Syk Kinase
MH  - Umbilical Cord/cytology
MH  - Virulence Factors, Bordetella/pharmacology
MH  - p38 Mitogen-Activated Protein Kinases
MH  - src-Family Kinases/drug effects/metabolism
EDAT- 2001/01/12 11:00
MHDA- 2001/03/17 10:01
CRDT- 2001/01/12 11:00
PHST- 2001/01/12 11:00 [pubmed]
PHST- 2001/03/17 10:01 [medline]
PHST- 2001/01/12 11:00 [entrez]
AID - S0006-4971(20)65997-3 [pii]
AID - 10.1182/blood.v97.2.359 [doi]
PST - ppublish
SO  - Blood. 2001 Jan 15;97(2):359-66. doi: 10.1182/blood.v97.2.359.