PMID- 11155355
OWN - NLM
STAT- MEDLINE
DCOM- 20010308
LR  - 20061115
IS  - 0001-723X (Print)
IS  - 0001-723X (Linking)
VI  - 44
IP  - 3
DP  - 2000 Jun-Aug
TI  - Detection of lymphotropic herpesvirus DNA by polymerase chain reaction in 
      cerebrospinal fluid of AIDS patients with neurological disease.
PG  - 137-43
AB  - Cerebrospinal fluid (CSF) samples from 49 acquired immunodefficiency disease 
      syndrome (AIDS) patients with a central nervous system (CNS) disease were 
      examined by polymerase chain reaction (PCR) to evaluate the association between 
      the positivity for cytomegalovirus (CMV) and Epstein-Barr virus (EBV), and 
      clinical diagnosis of a CNS disease. Frequency and clinical relevance of 
      detection of DNA of human herpesviruses 6 (HHV-6), 7 (HHV-7) and 8 (HHV-8) were 
      also determined. DNA of one or more of the following viruses was found in 26 of 
      49 patients (53%): CMV in 16 (33%), EBV in 13 (27%), human herpesvirus 6 (HHV-6) 
      in 2 (4%), human herpesvirus 7 (HHV-7) in 1 (2%), and human herpesvirus 8 (HHV-8) 
      in 1 (2%). The CMV detection was significantly associated with encephalitis and 
      peripheral neuropathy (7/16 vs. 2/33, p = 0.003), while EBV with primary CNS 
      lymphoma (P-CNSL) (8/13 vs. 0/36, p < 0.0001). HHV-6 DNA was found in CSF of two 
      patients with neuroradiological features suggestive of cerebral lesions. HHV-8 or 
      HHV-7 DNA was detected in the CSF of patients with unexplained neurological 
      symptoms. This study confirms that the PCR analysis of CSF is a valid tool for 
      the diagnosis of neurological diseases associated with CMV and EBV. On the other 
      hand, HHV-6, HHV-7 and HHV-8, instead, were rarely detected in CSF of AIDS 
      patients and have certainly no correlation with the CNS disease found.
FAU - Broccolo, F
AU  - Broccolo F
AD  - Department of Experimental Pathology, Medical Biotechnology, Infectious Diseases 
      and Epidemiology, University of Pisa, 56127 Pisa, Italy. 
      broccolo.francesco@hsr.it
FAU - Iuliano, R
AU  - Iuliano R
FAU - Careddu, A M
AU  - Careddu AM
FAU - Trovato, R
AU  - Trovato R
FAU - Lico, S
AU  - Lico S
FAU - Blanc, P L
AU  - Blanc PL
FAU - Mazzotta, F
AU  - Mazzotta F
FAU - Ceccherini-Nelli, L
AU  - Ceccherini-Nelli L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Slovakia
TA  - Acta Virol
JT  - Acta virologica
JID - 0370401
RN  - 0 (DNA, Viral)
SB  - IM
MH  - AIDS-Related Opportunistic Infections/cerebrospinal fluid/*virology
MH  - Acquired Immunodeficiency Syndrome/*complications
MH  - Central Nervous System Diseases/cerebrospinal fluid/*complications/virology
MH  - Cytomegalovirus/genetics/*isolation & purification
MH  - Cytomegalovirus Infections/cerebrospinal fluid/*complications/virology
MH  - DNA, Viral/*cerebrospinal fluid
MH  - Encephalitis, Viral/complications/virology
MH  - Epstein-Barr Virus Infections/cerebrospinal fluid/complications/virology
MH  - Herpesvirus 4, Human/genetics/isolation & purification
MH  - Herpesvirus 6, Human/genetics/isolation & purification
MH  - Herpesvirus 7, Human/genetics/isolation & purification
MH  - Herpesvirus 8, Human/genetics/isolation & purification
MH  - Humans
MH  - Lymphoma, AIDS-Related/complications/virology
MH  - Peripheral Nervous System Diseases/complications/virology
MH  - Polymerase Chain Reaction
EDAT- 2001/01/13 11:00
MHDA- 2001/03/10 10:01
CRDT- 2001/01/13 11:00
PHST- 2001/01/13 11:00 [pubmed]
PHST- 2001/03/10 10:01 [medline]
PHST- 2001/01/13 11:00 [entrez]
PST - ppublish
SO  - Acta Virol. 2000 Jun-Aug;44(3):137-43.