PMID- 11155666
OWN - NLM
STAT- MEDLINE
DCOM- 20010301
LR  - 20041117
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 111
IP  - 9
DP  - 1998 Sep
TI  - Cathepsin B on invasion and metastasis of gastric carcinoma.
PG  - 784-8
AB  - OBJECTIVE: To investigate the expression of cathepsin B (CB) in human gastric 
      carcinoma tissue. METHODS: The expression of CB in human gastric tissue was 
      studied by using monospecific polyclonal rabbit antibody raised against human 
      liver CB for immunohistochemistry, and full length cDNA of CB for in situ 
      hybridization and dot blot. RESULTS: CB overexpression in gastric carcinoma was 
      found when compared with non-neoplastic gastric tissue at both mRNA and protein 
      levels. Diffuse cytoplasmic CB staining of mRNA and protein were identified in 
      malignant cells of 53.3% and 69.1% of gastric adenocarcinoma respectively. The 
      increased staining of CB in malignant cells was associated with the depth of the 
      invasiveness and growth pattern as well as metastasis of lymph nodes, but not 
      with the histological classification. It was also found that there were the 
      expression of CB in stromal cells of the tumor and the expression localized 
      mainly in the endothelial cells of the microvessels which correlated with 
      angiogenesis. CONCLUSION: These results indicate that the expression of CB in 
      gastric carcinoma is related to tumor progression, and leads to development of 
      the invasive phenotype.
FAU - Liu, Y
AU  - Liu Y
AD  - Shanghai Institute of Digestive Disease, Shanghai Second Medical University, 
      Shanghai 200001, China.
FAU - Xiao, S
AU  - Xiao S
FAU - Shi, Y
AU  - Shi Y
FAU - Wang, L
AU  - Wang L
FAU - Ren, W
AU  - Ren W
FAU - Sloane, B F
AU  - Sloane BF
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.22.1 (Cathepsin B)
SB  - IM
MH  - Adenocarcinoma/*metabolism/secondary
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cathepsin B/*biosynthesis/genetics
MH  - Female
MH  - Humans
MH  - Lymphatic Metastasis
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Stomach Neoplasms/*metabolism/pathology
EDAT- 2001/01/13 11:00
MHDA- 2001/03/07 10:01
CRDT- 2001/01/13 11:00
PHST- 2001/01/13 11:00 [pubmed]
PHST- 2001/03/07 10:01 [medline]
PHST- 2001/01/13 11:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 1998 Sep;111(9):784-8.