PMID- 11155742
OWN - NLM
STAT- MEDLINE
DCOM- 20010125
LR  - 20161013
IS  - 0929-6646 (Print)
IS  - 0929-6646 (Linking)
VI  - 99
IP  - 12
DP  - 2000 Dec
TI  - Frequency and characterization of platelet-specific antibodies in patients who 
      received multiple platelet transfusions.
PG  - 902-5
AB  - BACKGROUND AND PURPOSE: Multiple platelet transfusions may induce 
      alloimmunization. Platelet alloimmunization involves human leukocyte antigen 
      (HLA) antibodies and platelet-specific antibodies, which recognize platelet 
      glycoproteins. No data are available about the frequency and characteristics of 
      platelet-specific antibodies in patients who have received multiple platelet 
      transfusions in Taiwan. METHODS: From June through December 1998, blood samples 
      from 103 patients who had received multiple platelet transfusions were submitted 
      to our laboratory for examination of platelet antibodies. The samples were first 
      screened for HLA antibodies or platelet-specific antibodies using a solid phase 
      method. Positive sera were further tested using an enzyme-linked immunosorbent 
      assay (ELISA) to identify the specificities of the alloantibodies. RESULTS: Forty 
      of the 103 patients who had received multiple platelet transfusions were positive 
      for platelet-reactive antibodies. Further study using ELISA showed that 22 
      patients had both HLA and platelet-specific antibodies, 12 patients had HLA 
      antibodies alone, and five patients produced platelet-specific antibodies in the 
      absence of HLA antibodies. Most platelet-specific antibodies were found among 
      patients who had HLA antibodies: 81% of platelet-specific antibodies were found 
      in HLA-immunized patients. The most frequently involved platelet glycoprotein 
      antigens were Ia/IIa and IIb/IIIa. CONCLUSIONS: HLA antibodies were the most 
      frequently found platelet-reactive antibodies in Taiwanese patients who had 
      received multiple transfusions, although platelet-specific antibodies were also 
      common. The frequency of platelet-specific antibodies was not as low as 
      previously reported.
FAU - Lo, S C
AU  - Lo SC
AD  - Department of Laboratory Medicine, National Taiwan University Hospital, 7 
      Chung-Shan South Road, Taipei, Taiwan.
FAU - Lin, D T
AU  - Lin DT
FAU - Lin, S W
AU  - Lin SW
FAU - Chang, J S
AU  - Chang JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - J Formos Med Assoc
JT  - Journal of the Formosan Medical Association = Taiwan yi zhi
JID - 9214933
RN  - 0 (HLA Antigens)
RN  - 0 (Isoantibodies)
RN  - 0 (Platelet Membrane Glycoproteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibody Specificity
MH  - Blood Platelets/*immunology
MH  - Child
MH  - Child, Preschool
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - HLA Antigens/immunology
MH  - Humans
MH  - Infant
MH  - Isoantibodies/*blood
MH  - Male
MH  - Middle Aged
MH  - Platelet Membrane Glycoproteins/immunology
MH  - *Platelet Transfusion
EDAT- 2001/01/13 11:00
MHDA- 2001/02/28 10:01
CRDT- 2001/01/13 11:00
PHST- 2001/01/13 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2001/01/13 11:00 [entrez]
PST - ppublish
SO  - J Formos Med Assoc. 2000 Dec;99(12):902-5.