PMID- 11156521
OWN - NLM
STAT- MEDLINE
DCOM- 20010125
LR  - 20061115
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 90
IP  - 6
DP  - 2000 Dec 25
TI  - Detection of chromosomal aneusomy by fluorescence in situ hybridization in 
      fine-needle aspirates from breast tumors: application to the preoperative 
      diagnosis of breast carcinoma.
PG  - 373-8
AB  - BACKGROUND: The authors studied the clinical usefulness of fluorescence in situ 
      hybridization (FISH) analysis of a numerical aberration of chromosomes (aneusomy) 
      using fine-needle aspiration (FNA) samples from patients with breast tumors in 
      the preoperative diagnosis of breast carcinoma. METHODS: FNA samples were 
      obtained from 176 breast tumors and were subjected to conventional cytology and 
      FISH analysis using the centromere probes for chromosomes 1, 11, and 17. Patients 
      with FNA samples that showed aneusomy in at least one of the three chromosomes 
      were diagnosed as positive. RESULTS: Histologic examination revealed 157 
      malignancies and 19 benign results (10 fibroadenomas, 6 intraductal papillomas, 1 
      intracystic papilloma, and 2 ADH). The sensitivity, specificity, and diagnostic 
      accuracy were 85.4%, 94.7%, and 86.4%, respectively, for cytology and 90.4%, 
      100%, and 91.5%, respectively, for FISH. Of 15 breast malignancies that were 
      diagnosed with indeterminate cytology, 13 were diagnosed as positive with FISH 
      analysis (sensitivity, 86.7%). CONCLUSIONS: The results demonstrate that the use 
      of FISH in the diagnosis of FNA samples has a diagnostic accuracy comparable to 
      conventional cytology and is useful in making a definitive diagnosis of 
      malignancy (100% specificity) in patients with indeterminate cytologic results, 
      suggesting that FISH diagnosis can be a good adjunct to conventional cytology.
FAU - Tsukamoto, F
AU  - Tsukamoto F
AD  - Department of Surgical Oncology, Osaka University Medical School, Suita, Japan.
FAU - Miyoshi, Y
AU  - Miyoshi Y
FAU - Koyama, H
AU  - Koyama H
FAU - Watatani, M
AU  - Watatani M
FAU - Sasa, M
AU  - Sasa M
FAU - Shiba, E
AU  - Shiba E
FAU - Takami, S
AU  - Takami S
FAU - Inazawa, J
AU  - Inazawa J
FAU - Noguchi, S
AU  - Noguchi S
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Aneuploidy
MH  - Biopsy, Needle
MH  - Breast Neoplasms/diagnosis/*genetics
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Sensitivity and Specificity
EDAT- 2001/01/13 11:00
MHDA- 2001/02/28 10:01
CRDT- 2001/01/13 11:00
PHST- 2001/01/13 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2001/01/13 11:00 [entrez]
AID - 10.1002/1097-0142(20001225)90:6<373::AID-CNCR8>3.3.CO;2-O [pii]
PST - ppublish
SO  - Cancer. 2000 Dec 25;90(6):373-8.