PMID- 11156736
OWN - NLM
STAT- MEDLINE
DCOM- 20010118
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 123
IP  - 2
DP  - 2000 Dec
TI  - Importance of using comparative genomic hybridization to improve detection of 
      chromosomal changes in childhood acute lymphoblastic leukemia.
PG  - 114-22
AB  - We used comparative genomic hybridization (CGH) and conventional cytogenetics 
      (CC) to define chromosomal changes and to evaluate the usefulness of CGH in 65 
      patients having childhood acute lymphoblastic leukemia (ALL). Subsequently, 
      fluorescence in situ hybridization (FISH) was used to evaluate the CGH and 
      cytogenetic results. Comparative genomic hybridization revealed DNA copy number 
      changes in 49 (75%) patients (including 7 patients with unsuccessful cytogenetics 
      and 2 patients with normal karyotype). A total of 85 losses and 195 gains were 
      detected. The most commonly gained chromosomes were 21 (35%), X (31%), 18 (27%), 
      10 (26%), 6 (25%), 17 (25%), 4 (23%), and 14 (22%). Losses were most frequently 
      observed on chromosomes 9p (18%) and 12p (11%). Other losses were detected on 
      chromosomes 13q (9%), 6q (9%), 7p (8%), and chromosome X (6%). Conventional 
      cytogenetics revealed chromosomal changes in 53 (82%) patients. The employment of 
      CGH and FISH together with CC analysis revealed chromosomal changes in 62 (95%) 
      of the childhood ALL patients investigated. The CGH completed CC results in 36 
      patients; in 9 patients, the changes escaped detection without using CGH. The 
      results of our study were compared to 6 other CGH studies previously reported. 
      Our observations underline the benefits of supplementing routine cytogenetic 
      investigation in childhood ALL by FISH and CGH, because small unbalanced changes 
      may escape detection when conventional cytogenetics is the only diagnostic method 
      used.
FAU - Jarosova, M
AU  - Jarosova M
AD  - Department of Hematology/Oncology, Palacky University Hospital, Olomouc, Czech 
      Republic. marie.jarosova@fnol.cz
FAU - Holzerova, M
AU  - Holzerova M
FAU - Jedlickova, K
AU  - Jedlickova K
FAU - Mihal, V
AU  - Mihal V
FAU - Zuna, J
AU  - Zuna J
FAU - Stary, J
AU  - Stary J
FAU - Pospisilova, D
AU  - Pospisilova D
FAU - Zemanova, Z
AU  - Zemanova Z
FAU - Trka, J
AU  - Trka J
FAU - Blazek, J
AU  - Blazek J
FAU - Pikalova, Z
AU  - Pikalova Z
FAU - Indrak, K
AU  - Indrak K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Cytogenetic Analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Nucleic Acid Hybridization/*methods
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics/pathology
EDAT- 2001/01/13 11:00
MHDA- 2001/02/28 10:01
CRDT- 2001/01/13 11:00
PHST- 2001/01/13 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2001/01/13 11:00 [entrez]
AID - S0165460800003101 [pii]
AID - 10.1016/s0165-4608(00)00310-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2000 Dec;123(2):114-22. doi: 
      10.1016/s0165-4608(00)00310-1.