PMID- 11157968 OWN - NLM STAT- MEDLINE DCOM- 20010329 LR - 20181113 IS - 0021-9193 (Print) IS - 1098-5530 (Electronic) IS - 0021-9193 (Linking) VI - 183 IP - 4 DP - 2001 Feb TI - Multiple regulatory mechanisms act on the 5' untranslated region of the S-layer gene from Thermus thermophilus HB8. PG - 1491-4 AB - The role of the 5' untranslated region (5'UTR) of the S-layer gene from Thermus thermophilus was analyzed through the isolation of Delta 5'UTR mutants. In these mutants the half-life of splA mRNA was strongly reduced and slpA transcription was no longer subjected to growth phase-dependent repression. Overproduction and detachment of the external envelopes of the mutants were observed in stationary phase. FAU - Castan, P AU - Castan P AD - Departamento and Centro de Biologia Molecular Severo Ochoa, CSIC-UAM, CSIC Campus de Cantoblanco, Madrid 28049, Spain. FAU - de Pedro, M A AU - de Pedro MA FAU - Risco, C AU - Risco C FAU - Valles, C AU - Valles C FAU - Fernandez, L A AU - Fernandez LA FAU - Schwarz, H AU - Schwarz H FAU - Berenguer, J AU - Berenguer J LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Bacteriol JT - Journal of bacteriology JID - 2985120R RN - 0 (5' Untranslated Regions) RN - 0 (Bacterial Proteins) RN - 0 (Membrane Glycoproteins) RN - 0 (Membrane Proteins) RN - 0 (S-layer proteins) RN - 0 (surface layer protein A, Bacteria) SB - IM MH - 5' Untranslated Regions/*genetics MH - Bacterial Proteins/*genetics MH - Base Sequence MH - Gene Expression Regulation, Bacterial MH - Genes, Bacterial MH - *Membrane Glycoproteins MH - Membrane Proteins/*genetics MH - Molecular Sequence Data MH - Sequence Deletion MH - Thermus thermophilus/cytology/*genetics PMC - PMC95029 EDAT- 2001/02/07 11:00 MHDA- 2001/04/03 10:01 PMCR- 2001/02/01 CRDT- 2001/02/07 11:00 PHST- 2001/02/07 11:00 [pubmed] PHST- 2001/04/03 10:01 [medline] PHST- 2001/02/07 11:00 [entrez] PHST- 2001/02/01 00:00 [pmc-release] AID - 1355 [pii] AID - 10.1128/JB.183.4.1491-1494.2001 [doi] PST - ppublish SO - J Bacteriol. 2001 Feb;183(4):1491-4. doi: 10.1128/JB.183.4.1491-1494.2001.