PMID- 11158540
OWN - NLM
STAT- MEDLINE
DCOM- 20030328
LR  - 20190514
IS  - 1040-4651 (Print)
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Linking)
VI  - 13
IP  - 1
DP  - 2001 Jan
TI  - Chloroplast ribosomal protein S7 of Chlamydomonas binds to chloroplast mRNA 
      leader sequences and may be involved in translation initiation.
PG  - 207-18
AB  - Certain mutations isolated in the 5' untranslated region (5'UTR) of the 
      chloroplast rps7 gene in Chlamydomonas reduce expression of reporter genes. 
      Second site suppressors in this 5'UTR sequence restore reporter expression. 5'UTR 
      sequences with the original mutations fail to bind a 20-kD protein, one of five 
      proteins that bind to leaders of several chloroplast genes. However, 5'UTRs from 
      suppressed mutants restore binding to this protein but do not bind a 47-kD 
      protein present on the wild type and the original mutant 5'UTRs. The 20-kD 
      protein was shown to be the S7 protein of the chloroplast ribosomal small subunit 
      encoded by rps7, whereas the 47-kD protein was shown to be RB47, a poly(A) 
      binding protein. Our data are consistent with the hypothesis that the S7 protein 
      plays either a general or a specific regulatory role in translation initiation in 
      the chloroplast.
FAU - Fargo, D C
AU  - Fargo DC
AD  - Developmental, Cell and Molecular Biology Group, Duke University LSRC Building, 
      Box 91000 Research Drive, Durham, North Carolina 27708, USA. dcf2@duke.edu
FAU - Boynton, J E
AU  - Boynton JE
FAU - Gillham, N W
AU  - Gillham NW
LA  - eng
GR  - GM-19427/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Ribosomal Proteins)
RN  - 0 (ribosomal protein S7)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Animals
MH  - Base Sequence
MH  - Chlamydomonas/*metabolism
MH  - Chloroplasts/*genetics
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - Protein Binding
MH  - Protein Biosynthesis/*physiology
MH  - RNA, Messenger/chemistry/*genetics
MH  - Recombinant Proteins/metabolism
MH  - Ribosomal Proteins/*metabolism/physiology
PMC - PMC102210
EDAT- 2001/02/07 11:00
MHDA- 2003/03/29 05:00
PMCR- 2002/01/01
CRDT- 2001/02/07 11:00
PHST- 2001/02/07 11:00 [pubmed]
PHST- 2003/03/29 05:00 [medline]
PHST- 2001/02/07 11:00 [entrez]
PHST- 2002/01/01 00:00 [pmc-release]
AID - 00-0285 [pii]
AID - 10.1105/tpc.13.1.207 [doi]
PST - ppublish
SO  - Plant Cell. 2001 Jan;13(1):207-18. doi: 10.1105/tpc.13.1.207.