PMID- 11158987
OWN - NLM
STAT- MEDLINE
DCOM- 20010322
LR  - 20200930
IS  - 0363-6135 (Print)
IS  - 0363-6135 (Linking)
VI  - 280
IP  - 2
DP  - 2001 Feb
TI  - Increased L-arginine uptake and inducible nitric oxide synthase activity in 
      aortas of rats with heart failure.
PG  - H859-67
AB  - L-Arginine crosses the cell membrane primarily through the system y(+) 
      transporter. The aim of this study was to investigate the role of L-arginine 
      transport in nitric oxide (NO) production in aortas of rats with heart failure 
      induced by myocardial infarction. Tumor necrosis factor-alpha levels in aortas of 
      rats with heart failure were six times higher than in sham rats (P < 0.01). 
      L-Arginine uptake was increased in aortas of rats with heart failure compared 
      with sham rats (P < 0.01). Cationic amino acid transporter-2B and inducible (i) 
      nitric oxide synthase (NOS) expression were increased in aortas of rats with 
      heart failure compared with sham rats (P < 0.05). Aortic strips from rats with 
      heart failure treated with L-arginine but not D-arginine increased NO production 
      (P < 0.05). The effect of L-arginine on NO production was blocked by L-lysine, a 
      basic amino acid that shares the same system y(+) transporter with L-arginine, 
      and by the NOS inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME). Treatment 
      with L-lysine and L-NAME in vivo decreased plasma nitrate and nitrite levels in 
      rats with heart failure (P < 0.05). Our data demonstrate that NO production is 
      dependent on iNOS activity and L-arginine uptake and suggest that L-arginine 
      transport plays an important role in enhanced NO production in heart failure.
FAU - Stathopulos, P B
AU  - Stathopulos PB
AD  - Cardiology Research Laboratory, Departments of Medicine, Pharmacology, and 
      Toxicology, London Health Sciences Centre, University of Western Ontario, London, 
      Ontario, Canada N6A 4G5.
FAU - Lu, X
AU  - Lu X
FAU - Shen, J
AU  - Shen J
FAU - Scott, J A
AU  - Scott JA
FAU - Hammond, J R
AU  - Hammond JR
FAU - McCormack, D G
AU  - McCormack DG
FAU - Arnold, J M
AU  - Arnold JM
FAU - Feng, Q
AU  - Feng Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol Heart Circ Physiol
JT  - American journal of physiology. Heart and circulatory physiology
JID - 100901228
RN  - 0 (DNA Primers)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 10028-17-8 (Tritium)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Aorta/*enzymology
MH  - Arginine/*pharmacokinetics
MH  - Blood Pressure/physiology
MH  - DNA Primers
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Endothelium, Vascular/drug effects/metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression Regulation, Enzymologic
MH  - Heart Failure/*metabolism
MH  - Heart Rate/physiology
MH  - Male
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Nitric Oxide Synthase/*genetics/*metabolism
MH  - Nitric Oxide Synthase Type II
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tritium
MH  - Tumor Necrosis Factor-alpha/genetics
MH  - Ventricular Pressure/physiology
EDAT- 2001/02/13 11:00
MHDA- 2001/03/27 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/03/27 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - 10.1152/ajpheart.2001.280.2.H859 [doi]
PST - ppublish
SO  - Am J Physiol Heart Circ Physiol. 2001 Feb;280(2):H859-67. doi: 
      10.1152/ajpheart.2001.280.2.H859.