PMID- 11159181
OWN - NLM
STAT- MEDLINE
DCOM- 20010405
LR  - 20240413
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 158
IP  - 2
DP  - 2001 Feb
TI  - Differential and sequential expression of multiple chemokines during elicitation 
      of allergic contact hypersensitivity.
PG  - 431-40
AB  - Regulation of chemokine-mediated leukocyte migration within inflammatory tissues 
      is a complex event that cannot be mimicked and analyzed in vitro. We therefore 
      investigated the role of macrophage- and T-lymphocyte-specific chemoattractants 
      involved in the positioning of immune effector cells during the elicitation phase 
      of contact hypersensitivity, a prototype of a T-lymphocyte-mediated immune 
      reaction. Serial sections of skin biopsies obtained from sensitized individuals 
      at distinct time intervals after epicutaneous application of allergens were 
      hybridized with anti-sense probes of a large panel of chemokines or 
      immunohistologically labeled with leukocyte-specific antibodies. Multifocal 
      expression of monocyte chemoattractant protein-1 (MCP-1) was already detected 
      after 6 hours in basal keratinocytes clearly preceding the infiltration of 
      monocytes and T cells. Increasing basal expression of MCP-1 and, in addition, of 
      regulated upon activation, normal T-cell expressed and secreted (RANTES) after 12 
      hours was accompanied by dermal expression of MCP-1, macrophage-derived 
      chemoattractant (MDC), and RANTES and paralleled by infiltration of mononuclear 
      cells into dermis and epidermis. Expression of the T-lymphocyte-specific 
      chemokines IP-10 and MIG in epidermis and dermis and of MDC, pulmonary and 
      activation-regulated chemokine (PARC), and thymus and activation-regulated 
      chemokine (TARC) exclusively in the dermis started after 12 hours reaching 
      maximum levels at 72 hours and was associated with infiltration of T cells into 
      the epidermal compartment. Our data provide evidence that migrating effector 
      cells encounter multiple chemoattractant signals in a complex spatial and 
      temporal pattern. In particular, keratinocytes contribute to the vigorous 
      immigration by sequential expression of MCP-1, RANTES, and interferon-inducible 
      protein-10 (IP-10) monokine induced by gamma interferon (MIG), indicating that 
      chemokine-mediated nonimmunological mechanisms precede and corroborate 
      antigen-specific mechanisms during elicitation of contact hypersensitivity.
FAU - Goebeler, M
AU  - Goebeler M
AD  - Department of Dermatology, University of Wurzburg Medical School, Wurzburg, 
      Germany.
FAU - Trautmann, A
AU  - Trautmann A
FAU - Voss, A
AU  - Voss A
FAU - Brocker, E V
AU  - Brocker EV
FAU - Toksoy, A
AU  - Toksoy A
FAU - Gillitzer, R
AU  - Gillitzer R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Allergens)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CCL17 protein, human)
RN  - 0 (CCL18 protein, human)
RN  - 0 (CD3 Complex)
RN  - 0 (CD68 antigen, human)
RN  - 0 (CXCL9 protein, human)
RN  - 0 (Chemokine CCL17)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokine CXCL9)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CC)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Allergens/administration & dosage/immunology
MH  - Antigens, CD/analysis
MH  - Antigens, Differentiation, Myelomonocytic/analysis
MH  - CD3 Complex/analysis
MH  - Chemokine CCL17
MH  - Chemokine CCL2/genetics
MH  - Chemokine CCL5/genetics
MH  - Chemokine CXCL10
MH  - Chemokine CXCL9
MH  - Chemokines/*genetics
MH  - Chemokines, CC/genetics
MH  - Chemokines, CXC/genetics
MH  - Dermatitis, Allergic Contact/*genetics/immunology
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Macrophages/immunology/metabolism
MH  - RNA, Messenger/drug effects/genetics/metabolism
MH  - Skin/immunology/metabolism/pathology
MH  - T-Lymphocytes/immunology/metabolism
MH  - Time Factors
PMC - PMC1850305
EDAT- 2001/02/13 11:00
MHDA- 2001/04/06 10:01
PMCR- 2001/08/01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/04/06 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
PHST- 2001/08/01 00:00 [pmc-release]
AID - S0002-9440(10)63986-7 [pii]
AID - 2519 [pii]
AID - 10.1016/s0002-9440(10)63986-7 [doi]
PST - ppublish
SO  - Am J Pathol. 2001 Feb;158(2):431-40. doi: 10.1016/s0002-9440(10)63986-7.