PMID- 11159527
OWN - NLM
STAT- MEDLINE
DCOM- 20010412
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 97
IP  - 4
DP  - 2001 Feb 15
TI  - Existence of escape mutant in HTLV-I tax during the development of adult T-cell 
      leukemia.
PG  - 987-93
AB  - Although Tax protein is the main target of cytotoxic T lymphocyte (CTL) on human 
      T-cell lymphotropic virus type I (HTLV-I)-infected cells, and Tax peptide 11 
      through 19 binding to HLA-A*02 has been shown to elicit a strong CTL response, 
      there are patients with adult T-cell leukemia (ATL) bearing HLA-A*02. To explore 
      whether there is genetic variation in HTLV-I tax that can escape CTL recognition 
      during the development of ATL, the HTLV-I tax gene was sequenced in 55 patients 
      with ATL, 61 patients with HTLV-I-associated myelopathy/tropical spastic 
      paraparesis (HAM/TSP), and 62 healthy carriers, and it was correlated with the 
      presence of HLA-A*02. First, a premature stop codon in the 5' half of the tax 
      gene that looses transactivation activity on the viral enhancer was observed in 3 
      patients with acute and 1 patient with chronic ATL. This stop codon was revealed 
      to emerge after the viral transmission to the patient from sequence analysis in 
      family members with ATL. Second, amino acid change in Tax peptide 11-19 was 
      observed in 3 patients with ATL. CTL assays demonstrated that this altered Tax 
      11-19 peptide, observed in ATL patients with HLA-A*02, was not recognized by Tax 
      11-19-specific CTL. Two patients with ATL had large deletions in tax by 
      sequencing, and 5 patients with ATL had deletions in HTLV-I by Southern blotting. 
      These findings suggest that at some stage of ATL development, HTLV-I-infected 
      cells that can escape the host immune system are selected and have a chance to 
      accumulate genetic alterations for further malignant transformation, leading to 
      acute ATL.
FAU - Furukawa, Y
AU  - Furukawa Y
AD  - Third Department of Internal Medicine and the Center for Chronic Viral Diseases, 
      Faculty of Medicine, Kagoshima University, Kagoshima, Japan. 
      furukawy@m2.kufm.kagoshima-u.ac.jp
FAU - Kubota, R
AU  - Kubota R
FAU - Tara, M
AU  - Tara M
FAU - Izumo, S
AU  - Izumo S
FAU - Osame, M
AU  - Osame M
LA  - eng
SI  - GENBANK/AB036372
SI  - GENBANK/AB036373
SI  - GENBANK/AB036374
SI  - GENBANK/AB036375
SI  - GENBANK/AB036376
SI  - GENBANK/AB036377
SI  - GENBANK/AB036378
SI  - GENBANK/AB036379
SI  - GENBANK/AB036380
SI  - GENBANK/AB036381
SI  - GENBANK/AB036382
SI  - GENBANK/AB036383
SI  - GENBANK/AB045399
SI  - GENBANK/AB045400
SI  - GENBANK/AB045401
SI  - GENBANK/AB045402
SI  - GENBANK/AB045403
SI  - GENBANK/AB045404
SI  - GENBANK/AB045405
SI  - GENBANK/AB045406
SI  - GENBANK/AB045407
SI  - GENBANK/AB045408
SI  - GENBANK/AB045409
SI  - GENBANK/AB045410
SI  - GENBANK/AB045411
SI  - GENBANK/AB045412
SI  - GENBANK/AB045413
SI  - GENBANK/AB045414
SI  - GENBANK/AB045415
SI  - GENBANK/AB045416
SI  - GENBANK/AB045417
SI  - GENBANK/AB045418
SI  - GENBANK/AB045419
SI  - GENBANK/AB045420
SI  - GENBANK/AB045421
SI  - GENBANK/AB045422
SI  - GENBANK/AB045423
SI  - GENBANK/AB045424
SI  - GENBANK/AB045425
SI  - GENBANK/AB045426
SI  - GENBANK/AB045427
SI  - GENBANK/AB045428
SI  - GENBANK/AB045429
SI  - GENBANK/AB045430
SI  - GENBANK/AB045431
SI  - GENBANK/AB045432
SI  - GENBANK/AB045433
SI  - GENBANK/AB045434
SI  - GENBANK/AB045435
SI  - GENBANK/AB045436
SI  - GENBANK/AB045437
SI  - GENBANK/AB045438
SI  - GENBANK/AB045439
SI  - GENBANK/AB045440
SI  - GENBANK/AB045441
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Codon, Terminator)
RN  - 0 (Epitopes)
RN  - 0 (Gene Products, tax)
RN  - 0 (HLA-A2 Antigen)
SB  - IM
CIN - Blood. 2001 Feb 15;97(4):833. PMID: 11159502
MH  - *Codon, Terminator
MH  - DNA Mutational Analysis
MH  - Enhancer Elements, Genetic
MH  - Epitopes/genetics
MH  - Female
MH  - Gene Products, tax/chemistry/immunology
MH  - *Genes, pX
MH  - HLA-A2 Antigen/genetics/immunology
MH  - HTLV-I Infections/virology
MH  - Human T-lymphotropic virus 1/*genetics
MH  - Humans
MH  - *Immunologic Surveillance
MH  - Leukemia-Lymphoma, Adult T-Cell/immunology/*virology
MH  - Male
MH  - Molecular Sequence Data
MH  - Proviruses/genetics
MH  - Sequence Deletion
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Time Factors
MH  - Transcriptional Activation
EDAT- 2001/02/13 11:00
MHDA- 2001/04/17 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/04/17 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S0006-4971(20)56309-X [pii]
AID - 10.1182/blood.v97.4.987 [doi]
PST - ppublish
SO  - Blood. 2001 Feb 15;97(4):987-93. doi: 10.1182/blood.v97.4.987.