PMID- 11161034
OWN - NLM
STAT- MEDLINE
DCOM- 20040105
LR  - 20240109
IS  - 0032-0889 (Print)
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Linking)
VI  - 125
IP  - 2
DP  - 2001 Feb
TI  - The 5' end of the pea ferredoxin-1 mRNA mediates rapid and reversible 
      light-directed changes in translation in tobacco.
PG  - 770-8
AB  - Ferredoxin-1 (Fed-1) mRNA contains an internal light response element (iLRE) that 
      destabilizes mRNA when light-grown plants are placed in darkness. mRNAs 
      containing this element dissociate from polyribosomes in the leaves of transgenic 
      tobacco (Nicotiana tabacum) plants transferred to the dark for 2 d. Here, we 
      report in vivo labeling experiments with a chloramphenicol acetyl transferase 
      mRNA fused to the Fed-1 iLRE. Our data indicate that the Fed-1 iLRE mediates a 
      rapid decline in translational efficiency and that iLRE-containing mRNAs 
      dissociate from polyribosomes within 20 min after plants are transferred to 
      darkness. Both events occur before the decline in mRNA abundance, and 
      polyribosome association is rapidly reversible if plants are re-illuminated. 
      These observations support a model in which Fed-1 mRNA in illuminated leaves is 
      stabilized by its association with polyribosomes, and/or by translation. In 
      darkness a large portion of the mRNA dissociates from polyribosomes and is 
      subsequently degraded. We also show that a significant portion of total tobacco 
      leaf mRNA is shifted from polyribosomal to non-polyribosomal fractions after 20 
      min in the dark, indicating that translation of other mRNAs is also rapidly 
      down-regulated in response to darkness. This class includes some, but not all, 
      cytoplasmic mRNAs encoding proteins involved in photosynthesis.
FAU - Hansen, E R
AU  - Hansen ER
AD  - Department of Botany, North Carolina State University, Raleigh, North Carolina 
      27695, USA.
FAU - Petracek, M E
AU  - Petracek ME
FAU - Dickey, L F
AU  - Dickey LF
FAU - Thompson, W F
AU  - Thompson WF
LA  - eng
GR  - GM43108/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Ferredoxins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - 5' Untranslated Regions/*genetics
MH  - Darkness
MH  - Ferredoxins/*genetics
MH  - Light
MH  - Pisum sativum/*genetics/radiation effects
MH  - Plant Leaves/genetics/metabolism
MH  - Plants, Genetically Modified/genetics/radiation effects
MH  - Polyribosomes/genetics
MH  - Protein Biosynthesis/*radiation effects
MH  - RNA, Messenger/*genetics
MH  - Nicotiana/*genetics/radiation effects
PMC - PMC64878
EDAT- 2001/02/13 11:00
MHDA- 2004/01/06 05:00
PMCR- 2002/02/01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2004/01/06 05:00 [medline]
PHST- 2001/02/13 11:00 [entrez]
PHST- 2002/02/01 00:00 [pmc-release]
AID - 6437 [pii]
AID - 10.1104/pp.125.2.770 [doi]
PST - ppublish
SO  - Plant Physiol. 2001 Feb;125(2):770-8. doi: 10.1104/pp.125.2.770.