PMID- 11161314
OWN - NLM
STAT- MEDLINE
DCOM- 20010426
LR  - 20161124
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 289
IP  - 2
DP  - 2001 Feb 15
TI  - Detection of individual phospholipids in lipid mixtures by matrix-assisted laser 
      desorption/ionization time-of-flight mass spectrometry: phosphatidylcholine 
      prevents the detection of further species.
PG  - 202-16
AB  - Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass 
      spectrometry is an established tool for the analysis of proteins, whereas it 
      gained by far less interest in the field of lipid analysis. This method works 
      well with phospholipids as well as organic cell extracts and provides high 
      sensitivity and reproducibility. The aim of the present paper is to extend our 
      previous studies to the analysis of lysophospholipids and phospholipid mixtures. 
      To study the suitability of MALDI-TOF mass spectrometry for the analysis of 
      lysophospholipids, different phospholipids like phosphatidylcholine, 
      phosphatidylethanolamine, phosphatidylserine, phosphatidic acid, and 
      phosphatidylinositol as well as their mixtures were digested with phospholipase 
      A(2). Positive and negative ion mass spectra of all phospholipids before and 
      after digestion were recorded. In all these cases, the molecular ions of the 
      expected digestion products could be detected and only a very small extent of 
      further fragmentation was observed. On the other hand, spectra of phospholipid 
      mixtures containing phosphatidylcholine were strongly dominated by 
      phosphatidylcholine and lysophosphatidylcholine signals, which prevented the 
      detection of further phospholipids even if those lipids were present in 
      comparable amounts. This is of paramount interest for the analysis of tissue and 
      cell extracts.
CI  - Copyright 2001 Academic Press.
FAU - Petkovic, M
AU  - Petkovic M
AD  - Institute of Medical Physics and Biophysics, Medical Faculty, University of 
      Leipzig, Liebigstrasse 27, D-04103 Leipzig, Germany. petm@medizin.uni-leipzig.de
FAU - Schiller, J
AU  - Schiller J
FAU - Muller, M
AU  - Muller M
FAU - Benard, S
AU  - Benard S
FAU - Reichl, S
AU  - Reichl S
FAU - Arnold, K
AU  - Arnold K
FAU - Arnhold, J
AU  - Arnhold J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Lipids)
RN  - 0 (Lysophosphatidylcholines)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Phospholipids)
RN  - EC 3.1.1.32 (Phospholipases A)
SB  - IM
MH  - Animals
MH  - Brain/metabolism
MH  - Lipids/*chemistry
MH  - Lysophosphatidylcholines/analysis/chemistry
MH  - Mass Spectrometry
MH  - Models, Chemical
MH  - Ovum/chemistry
MH  - Pancreas/chemistry
MH  - Phosphatidylcholines/analysis/*chemistry
MH  - Phospholipases A/chemistry
MH  - Phospholipids/*analysis
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
MH  - Swine
EDAT- 2001/02/13 11:00
MHDA- 2001/05/01 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/05/01 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S0003-2697(00)94926-1 [pii]
AID - 10.1006/abio.2000.4926 [doi]
PST - ppublish
SO  - Anal Biochem. 2001 Feb 15;289(2):202-16. doi: 10.1006/abio.2000.4926.