PMID- 11161442
OWN - NLM
STAT- MEDLINE
DCOM- 20010301
LR  - 20071114
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 206
IP  - 2
DP  - 2000 Dec 15
TI  - A mouse model for immunization with ex vivo virus-infected dendritic cells.
PG  - 107-15
AB  - Dendritic cells (DCs) have been demonstrated to be an important if not essential 
      inducer of cellular immune responses. The ability to grow these cells in vitro 
      may open up new avenues for protective immunizations. In this study we have 
      analyzed the virus-specific memory response generated following immunization with 
      ex vivo-infected bone marrow-derived dendritic cells. We demonstrate that mouse 
      DCs are efficiently infected with influenza virus but do not release infectious 
      progeny virus. Ex vivo-infected DCs secrete interleukin-12 (IL-12) and induce a 
      potent T helper (Th)1-like immune response when injected into mice. This was 
      demonstrated by the generation of cytotoxic T lymphocytes, the production of high 
      levels of gamma-interferon, and undetectable levels of IL-4 upon in vitro 
      restimulation of splenocytes from immunized animals. In addition, the 
      virus-specific antibody response is primarily of the IgG2a isotype, consistent 
      with the expansion of Th1 cells. Animals immunized with DCs infected with X-31 
      influenza virus and challenged with PR8 influenza virus cleared the infection 
      faster than animals not vaccinated. Thus, infected DCs efficiently activate the 
      cellular immune response and induce heterosubtypic immunity in mice.
CI  - Copyright 2000 Academic Press.
FAU - Lopez, C B
AU  - Lopez CB
AD  - Department of Microbiology, Mount Sinai School of Medicine, One Gustave L. Levy 
      Place, New York, New York, 10029, USA.
FAU - Fernandez-Sesma, A
AU  - Fernandez-Sesma A
FAU - Czelusniak, S M
AU  - Czelusniak SM
FAU - Schulman, J L
AU  - Schulman JL
FAU - Moran, T M
AU  - Moran TM
LA  - eng
GR  - 1P01A148204/PHS HHS/United States
GR  - 1R01AI4111/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Adoptive Transfer/*methods
MH  - Animals
MH  - Antigens, Viral/immunology
MH  - Bone Marrow Cells/immunology/pathology/virology
MH  - Cell Differentiation/immunology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/pathology/transplantation/*virology
MH  - Disease Models, Animal
MH  - Dogs
MH  - Female
MH  - Immunologic Memory
MH  - Influenza A virus/classification/*immunology/pathogenicity
MH  - Injections, Intraperitoneal
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Orthomyxoviridae Infections/immunology/pathology/virology
MH  - Recurrence
MH  - Species Specificity
MH  - Stem Cells/immunology/virology
MH  - T-Lymphocytes, Cytotoxic/immunology/virology
MH  - Th1 Cells/immunology/metabolism/virology
MH  - Virion/growth & development/pathogenicity
EDAT- 2001/02/13 11:00
MHDA- 2001/03/07 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/03/07 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S0008-8749(00)91736-2 [pii]
AID - 10.1006/cimm.2000.1736 [doi]
PST - ppublish
SO  - Cell Immunol. 2000 Dec 15;206(2):107-15. doi: 10.1006/cimm.2000.1736.