PMID- 11162244
OWN - NLM
STAT- MEDLINE
DCOM- 20010524
LR  - 20131121
IS  - 0969-9961 (Print)
IS  - 0969-9961 (Linking)
VI  - 8
IP  - 1
DP  - 2001 Feb
TI  - BDNF increases the number of axotomized rat retinal ganglion cells expressing 
      GAP-43, L1, and TAG-1 mRNA--a supportive role for nitric oxide?
PG  - 103-13
AB  - The death of neurons and the limited ability to activate growth-associated genes 
      prevent the restoration of lesioned fiber tracts in the adult mammalian CNS. 
      Here, we characterized the effects of the survival-promoting neurotrophin 
      brain-derived neurotrophic factor (BDNF) on mRNA expression of GAP-43, L1, TAG-1, 
      and SC-1 in axotomized and regenerating rat retinal ganglion cells (RGCs). BDNF 
      led to de novo upregulation of TAG-1 mRNA in axotomized RGCs and to a threefold 
      increase in the number of GAP-43 and L1 mRNA-expressing RGCs. SC-1 expression 
      remained unchanged. However, BDNF did not improve long-distance axon regeneration 
      into a peripheral nerve graft. Surprisingly, potentiating BDNF-mediated 
      neuroprotection by simultaneous administration of a spin trap or a NOS inhibitor 
      counteracted the BDNF-induced growth-associated gene expression. This led us to 
      hypothesize that the BDNF effects on GAP-43, L1, and TAG-1 mRNA expression are 
      mediated by a NO-dependent mechanism. In summary, our data support the idea that 
      survival and axon regeneration of lesioned CNS neurons can be regulated 
      independently.
CI  - Copyright 2001 Academic Press.
FAU - Klocker, N
AU  - Klocker N
AD  - Department of Neurology, University of Tubingen, Tubingen, 72076, Germany. 
      nikolaj.kloecker@uni-tuebingen.de
FAU - Jung, M
AU  - Jung M
FAU - Stuermer, C A
AU  - Stuermer CA
FAU - Bahr, M
AU  - Bahr M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurobiol Dis
JT  - Neurobiology of disease
JID - 9500169
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cell Adhesion Molecules, Neuronal)
RN  - 0 (Cntn2 protein, rat)
RN  - 0 (Contactin 2)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (GAP-43 Protein)
RN  - 0 (Leukocyte L1 Antigen Complex)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 0 (RNA, Messenger)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - *Axotomy
MH  - Brain-Derived Neurotrophic Factor/antagonists & inhibitors/*pharmacology
MH  - *Cell Adhesion Molecules, Neuronal
MH  - Cell Survival/drug effects
MH  - Contactin 2
MH  - Free Radical Scavengers
MH  - GAP-43 Protein/*metabolism
MH  - In Situ Hybridization
MH  - Leukocyte L1 Antigen Complex
MH  - Male
MH  - Membrane Glycoproteins/*metabolism
MH  - Nerve Regeneration/physiology
MH  - Neural Cell Adhesion Molecules/*metabolism
MH  - Nitric Oxide/*physiology
MH  - RNA, Messenger/*biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Retinal Ganglion Cells/*metabolism/transplantation
EDAT- 2001/02/13 11:00
MHDA- 2001/05/26 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/05/26 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S0969-9961(00)90329-2 [pii]
AID - 10.1006/nbdi.2000.0329 [doi]
PST - ppublish
SO  - Neurobiol Dis. 2001 Feb;8(1):103-13. doi: 10.1006/nbdi.2000.0329.