PMID- 11162363
OWN - NLM
STAT- MEDLINE
DCOM- 20010315
LR  - 20131121
IS  - 0014-4894 (Print)
IS  - 0014-4894 (Linking)
VI  - 96
IP  - 3
DP  - 2000 Nov
TI  - Trichomonas vaginalis: chromatin and mitotic spindle during mitosis.
PG  - 130-8
AB  - The mitotic phases and the changes that the chromatin and mitotic microtubules 
      undergo during mitosis in the sexually transmitted parasite Trichomonas vaginalis 
      are described. Parasites arrested in the gap 2 phase of the cell cycle by 
      nutrient starvation were induced to mitosis by addition of fresh whole medium. 
      [(3)H] Thymidine labeling of trichomonad parasites for 24 h showed that parasites 
      have at least four synchronic duplications after mitosis induction. Fixed or live 
      and acridine orange (AO)-stained trichomonads analyzed at different times during 
      mitosis by epifluorescence microscopy showed that mitosis took about 45 min and 
      is divided into five stages: prophase, metaphase, early and late anaphase, early 
      and late telophase, and cytokinesis. The AO-stained nucleus of live trichomonads 
      showed green (DNA) and orange (RNA) fluorescence, and the nucleic acid nature was 
      confirmed by DNase and RNase treatment, respectively. The chromatin appeared 
      partially condensed during interphase. At metaphase, it appeared as six condensed 
      chromosomes, as recently reported, which decondensed at anaphase and migrated to 
      the nuclear poles at telophase. In addition, small bundles of microtubules (as 
      hemispindles) were detected only in metaphase with the polyclonal antibody 
      anti-Entamoeba histolytica alpha-tubulin. This antibody showed that the 
      hemispindle and an atractophore-like structure seem to duplicate and polarize 
      during metaphase. In conclusion, T. vaginalis mitosis involves five mitotic 
      phases in which the chromatin undergoes different degrees of condensation, from 
      chromosomes to decondensed chromatin, and two hemispindles that are observed only 
      in the metaphase stage.
CI  - Copyright 2000 Academic Press.
FAU - Gomez-Conde, E
AU  - Gomez-Conde E
AD  - Departamento de Biomedicina Molecular, Centro de Investigacion y Estudios 
      Avanzados del Instituto Politecnico Nacional, Mexico, D.F., Mexico.
FAU - Mena-Lopez, R
AU  - Mena-Lopez R
FAU - Hernandez-Jauregui, P
AU  - Hernandez-Jauregui P
FAU - Gonzalez-Camacho, M
AU  - Gonzalez-Camacho M
FAU - Arroyo, R
AU  - Arroyo R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Parasitol
JT  - Experimental parasitology
JID - 0370713
RN  - 0 (Chromatin)
RN  - 0 (DNA, Protozoan)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Protozoan)
RN  - F30N4O6XVV (Acridine Orange)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Chromatin/*physiology/ultrastructure
MH  - DNA, Protozoan/analysis
MH  - Fluorescent Antibody Technique, Indirect
MH  - Fluorescent Dyes
MH  - Humans
MH  - Microscopy, Fluorescence
MH  - *Mitosis
MH  - RNA, Protozoan/analysis
MH  - Spindle Apparatus/*physiology/ultrastructure
MH  - Thymidine
MH  - Time Factors
MH  - Trichomonas vaginalis/*cytology/ultrastructure
EDAT- 2001/02/13 11:00
MHDA- 2001/03/17 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/03/17 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S001448940094551X [pii]
AID - 10.1006/expr.2000.4551 [doi]
PST - ppublish
SO  - Exp Parasitol. 2000 Nov;96(3):130-8. doi: 10.1006/expr.2000.4551.