PMID- 11162530
OWN - NLM
STAT- MEDLINE
DCOM- 20010322
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 280
IP  - 1
DP  - 2001 Jan 12
TI  - Molecular cloning of a novel human gene on chromosome 4p11 by immunoscreening of 
      an ovarian carcinoma cDNA library.
PG  - 401-6
AB  - In our efforts to identify immunoreactive antigens in ovarian cancer, we used the 
      method of immunoscreening of an ovarian carcinoma cDNA expression library with 
      ascites fluid from ovarian cancer patients. Among many positive clones, one was 
      found to contain partial sequence of a novel gene. By searching expressed 
      sequence tags (ESTs) and human genome project databases as well as by screening 
      other cDNA libraries and by RT-PCR strategies, we were able to obtain the 
      full-length cDNA sequence (1.4 kb) and establish the genomic organization of this 
      new gene. We also identified two alternatively spliced forms, encoding for 
      slightly different proteins. The longer form (1.4 kb) is predicted to encode for 
      a 27.6 kDa protein of 245 amino acids. The shorter form (1.3 kb) encodes for a 
      truncated protein of 20.7 kDa and 208 amino acids. These proteins are not 
      significantly homologous to any known protein in the GenBank database. This gene 
      is composed of nine exons and eight introns. By fluorescence in situ 
      hybridization (FISH), it was mapped to chromosome 4p11. This gene is highly 
      expressed in many tissues, including testis, brain, placenta, ovary, prostate, 
      and mammary gland. The high level expression of the shorter form is restricted to 
      the central nervous system, including brain, cerebellum, and spinal cord, 
      suggesting that this form may have a unique function in the central nervous 
      system.
CI  - Copyright 2001 Academic Press.
FAU - Luo, L Y
AU  - Luo LY
AD  - Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, 
      Ontario, M5G 1X5, Canada.
FAU - Soosaipillai, A
AU  - Soosaipillai A
FAU - Diamandis, E P
AU  - Diamandis EP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Antigens, Neoplasm/chemistry/*genetics
MH  - Base Sequence
MH  - Brain/metabolism
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 4
MH  - DNA, Complementary
MH  - Exons
MH  - Expressed Sequence Tags
MH  - Female
MH  - *Gene Library
MH  - Human Genome Project
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Introns
MH  - Karyotyping
MH  - Male
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Organ Specificity
MH  - Ovarian Neoplasms/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2001/02/13 11:00
MHDA- 2001/03/27 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/03/27 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S0006-291X(00)94126-3 [pii]
AID - 10.1006/bbrc.2000.4126 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2001 Jan 12;280(1):401-6. doi: 
      10.1006/bbrc.2000.4126.