PMID- 11162664
OWN - NLM
STAT- MEDLINE
DCOM- 20010315
LR  - 20041117
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 280
IP  - 5
DP  - 2001 Feb 9
TI  - Regulation of fibronectin expression and splicing in migrating epithelial cells: 
      migrating MDCK cells produce a lesser amount of, but more active, fibronectin.
PG  - 1262-8
AB  - Previously we have demonstrated that in MDCK epithelial cells not only 
      transforming growth factor-beta (TGF-beta) but also hepatocyte growth 
      factor/scatter factor (HGF/SF) regulates fibronectin (FN) splicing by increasing 
      the ratio of EDA-containing FN (EDA+ FN) mRNA to EDA-minus FN (EDA- FN) mRNA 
      (EDA+/EDA- ratio). EDA+ FN is known to be upregulated in tissues where cells 
      actively migrate, such as those during morphogenesis, wound healing, and 
      tumorigenesis. However, a direct association between cell migration and FN 
      splicing at the EDA region has never been investigated. In this work, we have 
      shown by using an in vitro wound migration assay that migrating epithelial cells 
      regulate FN production and splicing differently compared to nonmigrating cells. 
      Wounds were introduced as migration stimuli into the 10-day-old confluent cell 
      sheet, where the EDA+/EDA- ratio and FN mRNA expression levels were stable. In 
      migrating cells at the wound edge, the FN mRNA level decreased by 0.73-fold and 
      the EDA+/EDA- ratio increased by 1.32-fold when compared with nonmigrating cells 
      apart from the wound edge. HGF/SF significantly stimulated cell migration at the 
      wound edge and concomitantly decreased the FN mRNA level by 0.60-fold and 
      increased the EDA+/EDA- ratio by 1.84-fold in migrating cells. In nonmigrating 
      cells apart from the wound edge, FN mRNA expression and splicing were not 
      influenced by either wound stimulation or HGF/SF. EDA+ FN stimulates cell 
      migration more effectively than EDA- FN and thus is considered to be a more 
      active variant of FN. Taken together, migrating MDCK cells appear to regulate FN 
      mRNA expression and splicing to produce a lesser amount of, but more active, FN.
FAU - Inoue, T
AU  - Inoue T
AD  - Department of Pathology, Miyazaki Medical College, 5200 Kihara, Kiyotake, 
      Miyazaki, 889-1692, Japan.
FAU - Nabeshima, K
AU  - Nabeshima K
FAU - Shimao, Y
AU  - Shimao Y
FAU - Meng, J Y
AU  - Meng JY
FAU - Koono, M
AU  - Koono M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Fibronectins)
RN  - 0 (RNA, Messenger)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Movement/*physiology
MH  - Dogs
MH  - Epithelial Cells/cytology/drug effects/*metabolism
MH  - Fibronectins/*genetics
MH  - Gene Expression Regulation/drug effects
MH  - Hepatocyte Growth Factor/pharmacology
MH  - Humans
MH  - Kidney/cytology/drug effects/metabolism
MH  - RNA Splicing/drug effects/*genetics
MH  - RNA, Messenger/drug effects/genetics/metabolism
MH  - Time Factors
EDAT- 2001/02/13 11:00
MHDA- 2001/03/17 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/03/17 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S0006-291X(01)94264-0 [pii]
AID - 10.1006/bbrc.2001.4264 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2001 Feb 9;280(5):1262-8. doi: 
      10.1006/bbrc.2001.4264.