PMID- 11162781
OWN - NLM
STAT- MEDLINE
DCOM- 20010315
LR  - 20161124
IS  - 0041-008X (Print)
IS  - 0041-008X (Linking)
VI  - 170
IP  - 3
DP  - 2001 Feb 1
TI  - The time-dose-response relationship for elicitation of contact dermatitis in 
      isoeugenol allergic individuals.
PG  - 166-71
AB  - The elicitation response in allergic contact dermatitis is dose dependent, but 
      the time-concentration relationship for elicitation has not previously been 
      described. In this study 27 isoeugenol-sensitive patients participated in serial 
      dilution patch tests with isoeugenol and a double-blinded Repeated Open 
      Application Test (ROAT) using two concentrations of isoeugenol, 0.2 and 0.05%. 
      Seven controls without isoeugenol allergy were also included. The participants 
      applied 3.72 +/- 1.57 (mean +/- SD) mg/cm(2) of coded isoeugenol solutions twice 
      a day to a 3 x 3 cm(2) area on the volar aspect of the right and left arm, 
      respectively. For each test site the applications continued until a reaction 
      appeared or for a maximum of 28 days. The minimal criteria for a positive 
      reaction regarded as allergic contact dermatitis was persistent erythema at the 
      ROAT test site. All controls were negative and 16/24 (66.7%) of the included 
      isoeugenol-sensitive subjects showed a positive ROAT to the 0.2% solution within 
      the study period (Fisher's test, p = 0.0024). Ten of the positive patients also 
      reacted to the 0.05% solution. The median number of days until a positive 
      reaction to the 0.2% solution was 7 days and was 15 days for the 0.05% solution. 
      There was a highly significant correlation between the patients' patch test 
      threshold and the number of days until a positive ROAT. In conclusion, the time 
      until an isoeugenol allergic individual reacts in a ROAT depends on the 
      individual sensitivity as well as the exposure concentrations; for low 
      concentrations of the allergen or low degree of sensitivity, the allergic contact 
      dermatitis may develop after several weeks of exposure. Therefore, a negative 
      ROAT after 7 days may be a false negative.
CI  - Copyright 2001 Academic Press.
FAU - Andersen, K E
AU  - Andersen KE
AD  - Department of Dermatology, Odense University Hospital, University of Southern 
      Denmark, Odense, Denmark.
FAU - Johansen, J D
AU  - Johansen JD
FAU - Bruze, M
AU  - Bruze M
FAU - Frosch, P J
AU  - Frosch PJ
FAU - Goossens, A
AU  - Goossens A
FAU - Lepoittevin, J P
AU  - Lepoittevin JP
FAU - Rastogi, S
AU  - Rastogi S
FAU - White, I
AU  - White I
FAU - Menne, T
AU  - Menne T
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Allergens)
RN  - 0 (Perfume)
RN  - 3T8H1794QW (Eugenol)
RN  - 5M0MWY797U (isoeugenol)
SB  - IM
MH  - Administration, Topical
MH  - Allergens/*adverse effects
MH  - Denmark
MH  - Dermatitis, Contact/*etiology
MH  - Dose-Response Relationship, Drug
MH  - Double-Blind Method
MH  - Drug Hypersensitivity/*etiology
MH  - Eugenol/administration & dosage/*adverse effects/analogs & derivatives
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Patch Tests
MH  - *Perfume/adverse effects
MH  - Time Factors
EDAT- 2001/02/13 11:00
MHDA- 2001/03/17 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/03/17 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S0041-008X(00)99095-1 [pii]
AID - 10.1006/taap.2000.9095 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2001 Feb 1;170(3):166-71. doi: 10.1006/taap.2000.9095.