PMID- 11162871
OWN - NLM
STAT- MEDLINE
DCOM- 20010315
LR  - 20190819
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 156
IP  - 1
DP  - 2000 Dec 7
TI  - Sensitivity of the SRBC PFC assay versus ELISA for detection of immunosuppression 
      by TCDD and TCDD-like congeners.
PG  - 1-11
AB  - The splenic antibody plaque forming cell (PFC) assay is a widely used assay in 
      immunotoxicity testing. A recent revision of the Federal Insecticide, Fungicide 
      and Rodenticide (FIFRA) Immunotoxicity test guidelines by the EPA recommended 
      that either the PFC assay or the sheep red blood cell (SRBC) specific serum IgM 
      enzyme-linked immunosorbent assay (ELISA) be used to assess the primary humoral 
      response to SRBCs. The PFC assay quantifies the number of plasma cells in the 
      spleen producing SRBC-specific antibody, while the ELISA measures SRBC-specific 
      IgM antibody in the serum. Because these two assays measure different endpoints, 
      there is a need for comparison of their sensitivity and reliability. The purpose 
      of this project was to determine if these two assays are equally sensitive to 
      suppression of the SRBC response in B6C3F1 female mice. Female B6C3F1 mice were 
      given a single oral exposure to different doses of 
      2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or four TCDD-like congeners. One week 
      later, two sets of mice were immunized with SRBC. The first set was evaluated for 
      the PFC response and the second for the ELISA response, on day 4 or 5 
      post-immunization, respectively. The four TCDD-like congeners tested were: 
      1,2,3,7,8-pentachlorodibenzo-p-dioxin (PeCDD), 1,2,3,4,7-pentachlorodibenzofuran 
      (4PeCDF), 3,3',4,4',5-pentachlorobiphenyl (PCB126) and 
      2,3',4,4',5-pentachlrorbiphenyl (PCB118). The results were used to generate 
      dose-response curves for the determination of the ED(50) for TCDD and each 
      TCDD-like congener. For all chemicals tested, measuring the level of 
      SRBC-specific IgM antibody by ELISA was more sensitive than the PFC assay to 
      detect immunosuppression, as indicated by lower ED(50) values. These results 
      indicate that the SRBC-specific IgM ELISA is a more sensitive assay for detecting 
      the T-cell mediated immunotoxicity of dioxin-like chemicals in this rodent model.
FAU - Johnson, C W
AU  - Johnson CW
AD  - Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel 
      Hill, NC 27599, USA.
FAU - Williams, W C
AU  - Williams WC
FAU - Copeland, C B
AU  - Copeland CB
FAU - DeVito, M J
AU  - DeVito MJ
FAU - Smialowicz, R J
AU  - Smialowicz RJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Benzofurans)
RN  - 0 (Dibenzofurans, Polychlorinated)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
MH  - Animals
MH  - Benzofurans/toxicity
MH  - Body Weight/drug effects
MH  - Dibenzofurans, Polychlorinated
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Hemolytic Plaque Technique
MH  - Immunosuppressive Agents/*toxicity
MH  - Mice
MH  - Organ Size/drug effects
MH  - Polychlorinated Biphenyls/toxicity
MH  - Polychlorinated Dibenzodioxins/*analogs & derivatives/*toxicity
MH  - Sensitivity and Specificity
EDAT- 2001/02/13 11:00
MHDA- 2001/03/17 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/03/17 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S0300483X00003309 [pii]
AID - 10.1016/s0300-483x(00)00330-9 [doi]
PST - ppublish
SO  - Toxicology. 2000 Dec 7;156(1):1-11. doi: 10.1016/s0300-483x(00)00330-9.