PMID- 11163124
OWN - NLM
STAT- MEDLINE
DCOM- 20010405
LR  - 20190915
IS  - 0741-8329 (Print)
IS  - 0741-8329 (Linking)
VI  - 22
IP  - 3
DP  - 2000 Nov
TI  - Repeated ethanol administration induces short- and long-term changes in 
      enkephalin and dynorphin tissue concentrations in rat brain.
PG  - 165-71
AB  - Recently, we have shown that rats repeatedly treated with ethanol and/or cocaine 
      have decreased kappa-opioid receptor mRNA levels in the mesolimbic system. The 
      aim of the present study was to investigate the short- and long-term effects of 
      repeated ethanol administration on opioid peptide concentrations in brain tissue 
      of male Sprague-Dawley rats. Dynorphin B (1-13) (Dyn B) and 
      Met-enkephalinArg(6)Phe(7) (MEAP), endogenous ligands to kappa- and delta-opioid 
      receptors, respectively, were measured using radioimmunoassays. The rats were 
      given either ethanol [intraperitoneal (ip), twice daily, 2 g/kg bw/dose] or 
      saline for 13 consecutive days. Thirty minutes after the last ethanol dose on Day 
      13, the Dyn B tissue concentration was significantly decreased in the cingulate 
      cortex. The MEAP tissue concentration was decreased in the hippocampus 5 days 
      after the last ethanol injection as compared to saline-treated controls. 
      Furthermore, the Dyn B and the MEAP concentrations were increased in the 
      periaqueductal grey area (PAG) at this time point. Of particular interest were 
      the significant increases in Dyn B tissue concentrations found in the nucleus 
      accumbens (NAcc) at 30 min and at 21 days after the last ethanol dose. The 
      results suggest that repeated ethanol administration induces both short- and 
      long-term changes in the tissue concentrations of opioids in certain brain 
      regions associated with motivation and reward.
FAU - Lindholm, S
AU  - Lindholm S
AD  - Clinical Alcohol and Drug Addiction Research, Department of Clinical 
      Neuroscience, Magnus Huss, M4:02, Karolinska Hospital, Karolinska Institutet, 
      SE-171 76 Stockholm, Sweden. sara.lindholm@fyfa.ki.se
FAU - Ploj, K
AU  - Ploj K
FAU - Franck, J
AU  - Franck J
FAU - Nylander, I
AU  - Nylander I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Alcohol
JT  - Alcohol (Fayetteville, N.Y.)
JID - 8502311
RN  - 0 (Endorphins)
RN  - 3K9958V90M (Ethanol)
RN  - 58569-55-4 (Enkephalin, Methionine)
RN  - 73024-95-0 (enkephalin-Met, Arg(6)-Phe(7)-)
RN  - 74913-18-1 (Dynorphins)
RN  - 83335-41-5 (rimorphin)
SB  - IM
MH  - Animals
MH  - Brain/*drug effects/*metabolism
MH  - Brain Chemistry
MH  - Dynorphins/analysis/*metabolism
MH  - Endorphins/analysis/*metabolism
MH  - Enkephalin, Methionine/*analogs & derivatives/analysis/*metabolism
MH  - Ethanol/*administration & dosage
MH  - Hippocampus/drug effects/metabolism
MH  - Kinetics
MH  - Male
MH  - Nucleus Accumbens/drug effects/metabolism
MH  - Periaqueductal Gray/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2001/02/13 11:00
MHDA- 2001/04/06 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/04/06 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S0741-8329(00)00118-X [pii]
AID - 10.1016/s0741-8329(00)00118-x [doi]
PST - ppublish
SO  - Alcohol. 2000 Nov;22(3):165-71. doi: 10.1016/s0741-8329(00)00118-x.