PMID- 11164267
OWN - NLM
STAT- MEDLINE
DCOM- 20010521
LR  - 20190915
IS  - 0168-1656 (Print)
IS  - 0168-1656 (Linking)
VI  - 84
IP  - 3
DP  - 2000 Dec 28
TI  - RT-PCR ELISA method for the analysis of neurotrophin mRNA expression in brain and 
      peripheral tissues.
PG  - 259-72
AB  - The levels of nerve growth factor (NGF) and brain derived neurotrophic factor 
      (BDNF) in brain and periphery are susceptible to changes during development and 
      as result of different physiopathological conditions, such as stress and aging 
      and during the onset and progression of neurological and autoimmune diseases. 
      Despite the sensitive methods for measurement of neurotrophin protein levels in 
      different tissues, no easily applicable methods to evaluate changes in the level 
      of NGF and BDNF mRNA expression within physiological range have been described. 
      This study reports the development of a reproducible and simple procedure for 
      measurement of neurotrophin mRNA expression in brain and peripheral tissues based 
      upon an enzyme linked immunosorbent assay (ELISA) detection system of reverse 
      transcriptase-polymerase chain reaction (RT-PCR) products. The major advantages 
      of this RT-PCR ELISA procedure is to allow the co-amplification of diverse mRNAs 
      starting from small amounts of tissues; to contemporaneously test a large number 
      of samples; to be rapid and to use only commercial reagents and widely available 
      equipment. The procedure could also be useful in studies addressed to measure the 
      pattern of expression of molecules involved in the pathogenesis of 
      neurodegenerative and inflammatory diseases, such as neuropeptides and cytokines.
FAU - Tirassa, P
AU  - Tirassa P
AD  - Institute of Neurobiology (CNR), Viale C. Marx, 15/43, 00137, Rome, Italy.
FAU - Manni, L
AU  - Manni L
FAU - Stenfors, C
AU  - Stenfors C
FAU - Lundeberg, T
AU  - Lundeberg T
FAU - Aloe, L
AU  - Aloe L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Biotechnol
JT  - Journal of biotechnology
JID - 8411927
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - Brain-Derived Neurotrophic Factor/biosynthesis/genetics
MH  - Cerebral Cortex/metabolism
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Gene Amplification
MH  - Gene Expression Regulation
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
MH  - Hippocampus/metabolism
MH  - Mice
MH  - Myocardium/metabolism
MH  - Nerve Growth Factors/*biosynthesis/genetics
MH  - Organ Specificity/genetics
MH  - RNA, Messenger/*biosynthesis
MH  - Reproducibility of Results
MH  - *Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Skin/metabolism
MH  - Spleen/metabolism
MH  - Submandibular Gland/metabolism
EDAT- 2001/02/13 11:00
MHDA- 2001/05/25 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/05/25 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S0168165600003709 [pii]
AID - 10.1016/s0168-1656(00)00370-9 [doi]
PST - ppublish
SO  - J Biotechnol. 2000 Dec 28;84(3):259-72. doi: 10.1016/s0168-1656(00)00370-9.