PMID- 11164497
OWN - NLM
STAT- MEDLINE
DCOM- 20010524
LR  - 20191210
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 91
IP  - 2
DP  - 2001 Feb
TI  - HSV-2 disrupts gap junctional intercellular communication between mammalian cells 
      in vitro.
PG  - 157-66
AB  - Infection by herpes simplex virus-2 (HSV-2) disrupts both dye and electrical 
      coupling in Vero (African green monkey kidney) cell cultures. Vero cells in vitro 
      were iontophoretically injected with the fluorescent dye Lucifer yellow CH, the 
      spread of which revealed that cells throughout the confluent sheet shared open 
      gap junctions. However, 24 h after infection with the virus (but before cells 
      became rounded), dye always remained only within the target cell. Intracellular 
      electrophysiological measurements of ionic coupling revealed a 0.4 coupling 
      coefficient for adjacent cells in uninfected control cultures. By 3 h following 
      infection significant down-regulation of gap junctions had begun, preceding by 
      many hours any signs of infection visible with the light microscope. Measurements 
      between adjacent cells 3 h post-infection, a period when HSV-2 gene expression is 
      known to be at a maximum, yielded an average coupling coefficient of 0.35. By 6 h 
      post-infection (a period of known viral DNA replication) average coupling 
      coefficient for adjacent cells was 0.25, while by 24 h post-infection the average 
      fill still further to <0.08. A coupling coefficient of <0.08 suggests that 
      infection by HSV-2 completely disabled the gap junctions.
FAU - Fischer, N O
AU  - Fischer NO
AD  - Department of Biology, West Chester University, West Chester, PA 19383-8102, USA.
FAU - Mbuy, G N
AU  - Mbuy GN
FAU - Woodruff, R I
AU  - Woodruff RI
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Isoquinolines)
RN  - 9654F8OVKE (lucifer yellow)
SB  - IM
MH  - Animals
MH  - *Cell Communication
MH  - Chlorocebus aethiops
MH  - Electrophysiology
MH  - Fluorescent Dyes
MH  - *Gap Junctions/physiology
MH  - Herpesvirus 2, Human/*physiology
MH  - In Vitro Techniques
MH  - Isoquinolines
MH  - Microelectrodes
MH  - Microinjections
MH  - Time Factors
MH  - Vero Cells
EDAT- 2001/02/13 11:00
MHDA- 2001/05/26 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/05/26 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S0166093400002603 [pii]
AID - 10.1016/s0166-0934(00)00260-3 [doi]
PST - ppublish
SO  - J Virol Methods. 2001 Feb;91(2):157-66. doi: 10.1016/s0166-0934(00)00260-3.