PMID- 11164785 OWN - NLM STAT- MEDLINE DCOM- 20010607 LR - 20220321 IS - 0006-8993 (Print) IS - 0006-8993 (Linking) VI - 890 IP - 2 DP - 2001 Feb 2 TI - Localization of nuclear coactivators p300 and steroid receptor coactivator 1 in the rat hippocampus. PG - 197-202 AB - Cofactors (coactivators and corepressors) play an important role in mediating ligand-dependent transcription by steroid hormone receptors, but little has been reported about the distribution of coactivators in the brain. The present study represents the first immunohistochemical attempt to elucidate the localization of coactivators p300 and steroid receptor coactivator 1 (SRC-1) in the hippocampus of the rat. p300- and SRC-1-immunoreactivities were observed in CA1-CA4 pyramidal cell layers of the hippocampus and the granular cell layer of the dentate gyrus, and their localization was in the cell nucleus. Double-labeling immunohistochemistry of p300 and SRC-1 indicated that both coactivators were colocalized in the same cell. Antibodies against neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP) were used to identify whether neuron and glia contained p300 or SRC-1. Most p300- or SRC-1-containing cells showed NSE-immunoreactivity, but a limited number of these cells showed GFAP-immunoreactivity. These findings suggest that in the hippocampus p300 and SRC-1 synergistically play an important role in activating ligand-dependent transcription by steroid hormone receptor. FAU - Ogawa, H AU - Ogawa H AD - Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, 465 Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan. hogawa@basic.kpu-m.ac.jp FAU - Nishi, M AU - Nishi M FAU - Kawata, M AU - Kawata M LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Brain Res JT - Brain research JID - 0045503 RN - 0 (Glial Fibrillary Acidic Protein) RN - 0 (Nuclear Proteins) RN - 0 (Trans-Activators) RN - 0 (Transcription Factors) RN - EC 2.3.1.48 (E1A-Associated p300 Protein) RN - EC 2.3.1.48 (Ep300 protein, rat) RN - EC 2.3.1.48 (Histone Acetyltransferases) RN - EC 2.3.1.48 (Nuclear Receptor Coactivator 1) RN - EC 4.2.1.11 (Phosphopyruvate Hydratase) SB - IM MH - Animals MH - E1A-Associated p300 Protein MH - Gene Expression Regulation/physiology MH - Glial Fibrillary Acidic Protein/metabolism MH - Hippocampus/cytology/*metabolism MH - Histone Acetyltransferases MH - Immunohistochemistry MH - Male MH - Neurons/cytology/*metabolism MH - Nuclear Proteins/*analysis MH - Nuclear Receptor Coactivator 1 MH - Phosphopyruvate Hydratase/metabolism MH - Rats MH - Rats, Wistar MH - Trans-Activators/*analysis MH - Transcription Factors/*analysis/physiology EDAT- 2001/02/13 11:00 MHDA- 2001/06/08 10:01 CRDT- 2001/02/13 11:00 PHST- 2001/02/13 11:00 [pubmed] PHST- 2001/06/08 10:01 [medline] PHST- 2001/02/13 11:00 [entrez] AID - S0006-8993(00)03158-9 [pii] AID - 10.1016/s0006-8993(00)03158-9 [doi] PST - ppublish SO - Brain Res. 2001 Feb 2;890(2):197-202. doi: 10.1016/s0006-8993(00)03158-9.