PMID- 11164890
OWN - NLM
STAT- MEDLINE
DCOM- 20010315
LR  - 20190826
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 37
IP  - 11
DP  - 2000 Aug
TI  - Site A of the MCP-1 distal regulatory region functions as a transcriptional 
      modulator through the transcription factor NF1.
PG  - 623-32
AB  - The monocyte chemoattractant protein-1 (MCP-1) functions to recruit monocytes and 
      macrophages to areas of inflammation and is a prototypic chemokine subjected to 
      coordinate regulation by immunomodulatory agents. TNF mediated regulation of 
      MCP-1 occurs through a distal regulatory region located 2.5 kb upstream of the 
      transcriptional start site. Within this region are two NF-kB motifs that are each 
      critical for function. Site A, located within the distal regulatory region and 
      upstream of the kappaB elements is required for maximal induction by TNF. 
      However, unlike the kappaB elements and other MCP-1 regulatory elements, Site A 
      is constitutively occupied by factors in vivo. To better understand the nature of 
      Site A function, this report identified a Site A binding protein and provides a 
      functional analysis of the element in driving transcription. The results showed 
      that the transcription factor NF1/CTF binds to Site A both in vitro and in vivo. 
      While Site A has no transcriptional activity on its own, it was found to augment 
      the transcriptional activity of a GAL4-VP16 reporter system in an orientation and 
      position independent manner. Because NF1 is known to interact with factors that 
      modify nucleosomes, these results suggest a unique role for Site A in regulating 
      MCP-1 expression.
FAU - Kumar, S N
AU  - Kumar SN
AD  - Department of Microbiology and Immunology, Emory University School of Medicine, 
      1510 Clifton Road, Atlanta, GA 30322, USA.
FAU - Boss, J M
AU  - Boss JM
LA  - eng
GR  - CA74271/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (CTF-1 transcription factor)
RN  - 0 (Chemokine CCL2)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - CCAAT-Enhancer-Binding Proteins/*physiology
MH  - Chemokine CCL2/chemistry/*physiology
MH  - *Gene Expression Regulation
MH  - Mice
MH  - Molecular Sequence Data
MH  - NFI Transcription Factors
MH  - Transcription Factors/*physiology
MH  - Transcription, Genetic
EDAT- 2001/02/13 11:00
MHDA- 2001/03/17 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/03/17 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S0161589000000973 [pii]
AID - 10.1016/s0161-5890(00)00097-3 [doi]
PST - ppublish
SO  - Mol Immunol. 2000 Aug;37(11):623-32. doi: 10.1016/s0161-5890(00)00097-3.