PMID- 11166408
OWN - NLM
STAT- MEDLINE
DCOM- 20010510
LR  - 20220318
IS  - 0168-3659 (Print)
IS  - 0168-3659 (Linking)
VI  - 70
IP  - 1-2
DP  - 2001 Jan 29
TI  - Biodegradable polymeric micelles composed of doxorubicin conjugated PLGA-PEG 
      block copolymer.
PG  - 63-70
AB  - Biodegradable polymeric micelles containing doxorubicin in the core region were 
      prepared from a di-block copolymer composed of doxorubicin-conjugated 
      poly(DL-lactic-co-glycolic acid) (PLGA) and polyethyleneglycol (PEG). The 
      di-block copolymer of PLGA-PEG was first synthesized and the primary amino group 
      of doxorubicin was then conjugated to the terminal hydroxyl group of PLGA, which 
      had been pre-activated using p-nitrophenyl chloroformate. The resulting polymeric 
      micelles in aqueous solution were characterized by measurement of size, drug 
      loading, and critical micelle concentration. The micelles containing 
      chemically-conjugated doxorubicin exhibited a more sustained release profile than 
      PEG-PLGA micelles containing physically-entrapped doxorubicin. The cytotoxic 
      activity of the micelles against HepG2 cells was greater than free doxorubicin, 
      suggesting that the micelles containing conjugated doxorubicin were more 
      effectively taken up cellularly, by an endocytosis mechanism rather than by 
      passive diffusion. Confocal microscopic observation and flow cytometry analysis 
      supported the enhanced cellular uptake of the micelles.
FAU - Yoo, H S
AU  - Yoo HS
AD  - Department of Biological Sciences, Korea Advanced Institute of Science and 
      Technology, Taejon 305-701, South Korea.
FAU - Park, T G
AU  - Park TG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Control Release
JT  - Journal of controlled release : official journal of the Controlled Release 
      Society
JID - 8607908
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (Micelles)
RN  - 0 (poly(lactic-glycolic acid)-poly(ethyleneglycol) copolymer)
RN  - 34346-01-5 (Polyglactin 910)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 80168379AG (Doxorubicin)
SB  - IM
MH  - Antibiotics, Antineoplastic/*administration & dosage
MH  - Doxorubicin/*administration & dosage/pharmacokinetics/pharmacology
MH  - Flow Cytometry
MH  - Humans
MH  - Micelles
MH  - Polyethylene Glycols/*administration & dosage
MH  - Polyglactin 910/*administration & dosage
MH  - Tumor Cells, Cultured
EDAT- 2001/02/13 11:00
MHDA- 2001/05/22 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/05/22 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S0168365900003400 [pii]
AID - 10.1016/s0168-3659(00)00340-0 [doi]
PST - ppublish
SO  - J Control Release. 2001 Jan 29;70(1-2):63-70. doi: 10.1016/s0168-3659(00)00340-0.