PMID- 11166776
OWN - NLM
STAT- MEDLINE
DCOM- 20010419
LR  - 20191210
IS  - 0021-9150 (Print)
IS  - 0021-9150 (Linking)
VI  - 154
IP  - 2
DP  - 2001 Feb 1
TI  - Activation of the immune system and coronary artery disease: the role of 
      anti-endothelial cell antibodies.
PG  - 429-36
AB  - On the basis of the role of immuno-mediated inflammation in atherosclerosis we 
      investigated, (1) the prevalence of anti-endothelial cell antibodies (AECA) in 
      ischaemic heart disease (IHD); (2) if beta2-glycoprotein I (beta2-GPI) was the 
      target antigen of AECA; (3) the relationship between AECA, tissue factor (TF) and 
      tissue factor pathway inhibitor (TFPI). In 93 consecutive IHD patients undergoing 
      percutaneous transluminal coronary angioplasty (PTCA) and 105 controls AECA were 
      detected by ELISA on human umbilical vein endothelial cells (HUVEC). AECA 
      positive sera were evaluated for anti-beta2-GPI antibodies by ELISA. TF and TFPI 
      plasma levels were assessed by ELISA. Twelve of 93 (12.9%) IHD patients and only 
      one of 105 controls (0.95%) were AECA positive. The prevalence of AECA was higher 
      in unstable angina (UA) than in effort angina (EA) (P=0.01). Three of 12 AECA 
      positive sera resulted positive for anti-beta2-GPI and showed a marked decrease 
      in EC-binding when tested on HUVEC cultured in serum-free medium. The binding was 
      restored by the addition of beta2-GPI. TF and TFPI levels were similar in AECA 
      positive and AECA negative patients. The rate of angiographically documented 
      clinical recurrences was 66.7% in the AECA positive and 14.8% in the AECA 
      negative group (P=0.0004) with a significant relationship between restenosis and 
      AECA (P<0.0001), unchanged by the inclusion of cardiovascular risk factors in the 
      regression model. Our results suggest a 'role' for AECA in the immune-mediated 
      inflammation in UA beta2-GPI is not the only AECA target antigen. AECA are not 
      responsible for high TF and TFPI levels. The high rate of clinical recurrences 
      after PTCA, confirmed by angiography, in AECA positive patients is in line with 
      such a role and suggests further large-scale 'ad hoc' studies.
FAU - Farsi, A
AU  - Farsi A
AD  - Istituto di Clinica Medica Generale e Cardiologia, University of Florence, Viale 
      Morgagni 85, 50134, Florence, Italy.
FAU - Domeneghetti, M P
AU  - Domeneghetti MP
FAU - Brunelli, T
AU  - Brunelli T
FAU - Gori, A M
AU  - Gori AM
FAU - Fedi, S
AU  - Fedi S
FAU - Gensini, G F
AU  - Gensini GF
FAU - Giglioli, C
AU  - Giglioli C
FAU - Prisco, D
AU  - Prisco D
FAU - Passaleva, A
AU  - Passaleva A
FAU - Meroni, P L
AU  - Meroni PL
FAU - Del Papa, N
AU  - Del Papa N
FAU - Abbate, R
AU  - Abbate R
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (Apolipoproteins)
RN  - 0 (Autoantibodies)
RN  - 0 (Biomarkers)
RN  - 0 (Factor Xa Inhibitors)
RN  - 0 (Glycoproteins)
RN  - 0 (Lipoproteins)
RN  - 0 (anti-endothelial cell antibody)
RN  - 0 (beta 2-Glycoprotein I)
RN  - 0 (lipoprotein-associated coagulation inhibitor)
RN  - 9035-58-9 (Thromboplastin)
SB  - IM
MH  - Angioplasty, Balloon, Coronary
MH  - Apolipoproteins/immunology/metabolism
MH  - Autoantibodies/*immunology
MH  - Biomarkers
MH  - Coronary Angiography
MH  - Coronary Disease/diagnostic imaging/*immunology/metabolism/therapy
MH  - Disease Progression
MH  - Endothelium, Vascular/immunology/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Factor Xa Inhibitors
MH  - Female
MH  - Glycoproteins/immunology/metabolism
MH  - Humans
MH  - Lipoproteins/immunology/metabolism
MH  - Male
MH  - Middle Aged
MH  - Recurrence
MH  - Thromboplastin/immunology/metabolism
MH  - Umbilical Veins/metabolism
MH  - beta 2-Glycoprotein I
EDAT- 2001/02/13 11:00
MHDA- 2001/04/21 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/04/21 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - S0021-9150(00)00482-2 [pii]
AID - 10.1016/s0021-9150(00)00482-2 [doi]
PST - ppublish
SO  - Atherosclerosis. 2001 Feb 1;154(2):429-36. doi: 10.1016/s0021-9150(00)00482-2.