PMID- 11167353
OWN - NLM
STAT- MEDLINE
DCOM- 20010419
LR  - 20190822
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 56
IP  - 1
DP  - 2001 Jan
TI  - Interleukin-4-positive mast cells are highly associated with the extent of 
      immediate allergic wheal reaction in the skin.
PG  - 58-64
AB  - BACKGROUND: In addition to histamine, mast cells contain other potent mediators 
      which can contribute to the allergic wheal reaction in the skin. METHODS: To 
      study the association of tryptase-, chymase-, and interleukin-4 (IL-4)-positive 
      mast cells with the size of the prick-test wheal reaction, 50 sensitive atopic 
      subjects were prick-tested with the cow-dander allergen on the forearm skin, and 
      the wheal area was measured. A corresponding site of intact healthy-looking skin 
      was biopsied and examined enzyme-histochemically for tryptase and chymase. A 
      double-staining method was used to demonstrate the immunoreactivity of IL-4 and 
      chymase inhibitors (alpha1-proteinase inhibitor and alpha1-antichymotrypsin) in 
      mast cells. The levels of total and cow-specific immunoglobulin E (IgE) were 
      measured in serum. RESULTS: The number of tryptase- and chymase-positive mast 
      cells or those containing chymase inhibitors revealed no correlation with the 
      wheal reaction. In contrast, both the percentage and the number of IL-4-positive 
      mast cells showed significant positive correlation with the wheal size per se 
      (P<0.0001), as well as with the ratio of the wheal size by cow allergen to that 
      by histamine control (P<0.003). In addition, tryptase-, chymase-, and 
      IL-4-positive mast cells correlated with total IgE, but not with specific IgE, 
      levels, and they showed no relation to the clinical manifestation of atopic 
      disease, asthma or atopic dermatitis. CONCLUSIONS: The novel finding was that 
      IL-4-positive, but not tryptase- and chymase-positive, mast cells are intimately 
      associated with the extent of the prick-test wheal.
FAU - Saarinen, J V
AU  - Saarinen JV
AD  - Department of Dermatology, Kuopio University Hospital, Finland.
FAU - Harvima, R J
AU  - Harvima RJ
FAU - Naukkarinen, A
AU  - Naukkarinen A
FAU - Horsmanheimo, M
AU  - Horsmanheimo M
FAU - Harvima, I T
AU  - Harvima IT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (alpha 1-Antichymotrypsin)
RN  - 0 (alpha 1-Antitrypsin)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.39 (Chymases)
RN  - EC 3.4.21.59 (Tryptases)
SB  - IM
EIN - Allergy 2001 May;56(5):451
MH  - Adolescent
MH  - Adult
MH  - Biopsy
MH  - Cell Count
MH  - Chymases
MH  - Female
MH  - Humans
MH  - Hypersensitivity, Immediate/blood/diagnosis/*immunology
MH  - Immunoglobulin E/blood
MH  - Interleukin-4/*analysis
MH  - Male
MH  - Mast Cells/*chemistry/cytology/enzymology
MH  - Middle Aged
MH  - Serine Endopeptidases/analysis
MH  - Skin/pathology
MH  - Skin Tests
MH  - Tryptases
MH  - alpha 1-Antichymotrypsin/analysis
MH  - alpha 1-Antitrypsin/analysis
EDAT- 2001/02/13 11:00
MHDA- 2001/04/21 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/04/21 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - all833 [pii]
AID - 10.1034/j.1398-9995.2001.00833.x [doi]
PST - ppublish
SO  - Allergy. 2001 Jan;56(1):58-64. doi: 10.1034/j.1398-9995.2001.00833.x.