PMID- 11167995
OWN - NLM
STAT- MEDLINE
DCOM- 20010308
LR  - 20220410
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 123
IP  - 1
DP  - 2001 Jan
TI  - Hypoxia induces expression of the chemokines monocyte chemoattractant protein-1 
      (MCP-1) and IL-8 in human dermal fibroblasts.
PG  - 36-41
AB  - Hypoxia is an important factor in the pathophysiology of vascular and 
      inflammatory diseases. Leucocyte infiltration, as a consequence of adhesion 
      molecule up-regulation and chemokine release, is a prominent feature of these 
      diseases. The objective of our study was to investigate the potential role of 
      resident fibroblasts in hypoxia-induced chemotactic responses. We show that MCP-1 
      and IL-8 mRNA are specifically induced by hypoxia in dermal fibroblasts. This 
      response is paralleled by increased NF-kappaB p65/p50 binding activity, and it is 
      inhibited by pretreatment with N-acetyl-L-cysteine. MCP-1 secreted by fibroblasts 
      is chemotactic for monocytic cells and this activity is significantly increased 
      by hypoxia. Chemotactic index correlates with MCP-1 protein levels and is 
      significantly decreased by neutralizing anti-MCP-1 MoAb. These findings 
      demonstrate the ability of resident fibroblasts to mediate chemotaxis of 
      leucocytes through the release of chemokines in response to hypoxia. Our data 
      point to MCP-1 as an important component in this response, and therefore it may 
      be a potential target in inflammatory responses associated with hypoxia.
FAU - Galindo, M
AU  - Galindo M
AD  - Unidad de Investigacion, Hospital 12 de Octubre, Madrid, Spain. reuma@h12o.es
FAU - Santiago, B
AU  - Santiago B
FAU - Alcami, J
AU  - Alcami J
FAU - Rivero, M
AU  - Rivero M
FAU - Martin-Serrano, J
AU  - Martin-Serrano J
FAU - Pablos, J L
AU  - Pablos JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (NF-kappa B)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Cell Hypoxia/*immunology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/metabolism/physiology
MH  - Chemotaxis, Leukocyte/immunology
MH  - Fibroblasts/*immunology/metabolism
MH  - Humans
MH  - Interleukin-8/*biosynthesis
MH  - Monocytes/immunology
MH  - NF-kappa B/metabolism
MH  - Oxygen/metabolism
MH  - Skin/cytology/*immunology/metabolism
PMC - PMC1905951
EDAT- 2001/02/13 11:00
MHDA- 2001/03/10 10:01
PMCR- 2002/01/01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/03/10 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
PHST- 2002/01/01 00:00 [pmc-release]
AID - cei1412 [pii]
AID - 10.1046/j.1365-2249.2001.01412.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2001 Jan;123(1):36-41. doi: 10.1046/j.1365-2249.2001.01412.x.