PMID- 11169139
OWN - NLM
STAT- MEDLINE
DCOM- 20020107
LR  - 20191025
IS  - 0902-0055 (Print)
IS  - 0902-0055 (Linking)
VI  - 16
IP  - 1
DP  - 2001 Feb
TI  - HLA-DR4 and salivary immunoglobulin A reactions to oral streptococci.
PG  - 45-53
AB  - The aim of this study was to describe and compare salivary immunoglobulin A (IgA) 
      antibody reactions to extracts of strains of three oral streptococci in human 
      leukocyte antigen (HLA)-DR4-positive and -DR4-negative subjects. Whole 
      paraffin-stimulated saliva samples were collected from 27 apparently healthy 
      subjects. Previous HLA typing showed that 20 subjects were DR4 positive and 7 
      were DR4 negative. HLA-DRB1*04 subtyping was performed among the DR4-positive 
      subjects. Whole-cell antigen extracts from Streptococcus mutans (KPSK 2), 
      Streptococcus sobrinus (OMZ 65) and Streptococcus parasanguis (Nt 62) were 
      separated in SDS-PAGE. The antigens were immunoblotted with diluted saliva 
      (Western blot), scanned and analyzed in a computer system. All immunoblot bands 
      were recorded in DR4-positive and DR4-negative saliva pools, and bands with an 
      optical density >or=0.1 were selected for analysis in individual salivas. The 
      DR4-negative subjects in general had more immunoblot bands and more distinct 
      bands than did the DR4-positive subjects. A higher concentration of total IgA in 
      saliva was correlated with more bands, especially to antigens separated from S. 
      mutans. When the number of bands was calculated per IgA unit, significant 
      differences were observed between DR4-positive and DR4-negative salivas. This was 
      particularly seen for S. mutans and S. parasanguis. As the number of bands was 
      analyzed in relation to DR4 subgroups, DRB1*04, there was a lower salivary IgA 
      activity to S. mutans in the DRB1*0401 and *0404. The variable level of 
      correlation previously demonstrated for S. mutans colonisation and serologically 
      defined DR4 positive subjects might be explained by the heterogeneity in this 
      group, and the relation should be sought on a subgroup level.
FAU - Wallengren, M L
AU  - Wallengren ML
AD  - Department of Cariology, Faculty of Odontology, Malmo University, Sweden.
FAU - Ericson, D
AU  - Ericson D
FAU - Hamberg, K
AU  - Hamberg K
FAU - Johnson, U
AU  - Johnson U
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Oral Microbiol Immunol
JT  - Oral microbiology and immunology
JID - 8707451
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (HLA-DR4 Antigen)
RN  - 0 (Immunoglobulin A, Secretory)
RN  - 0 (Salivary Proteins and Peptides)
MH  - Antibodies, Bacterial/immunology
MH  - Antigen-Antibody Reactions/immunology
MH  - Antigens, Bacterial/immunology
MH  - Blotting, Western
MH  - Electrophoresis, Polyacrylamide Gel
MH  - HLA-DR4 Antigen/genetics/*immunology
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin A, Secretory/*immunology
MH  - Mouth/*microbiology
MH  - Saliva/*immunology
MH  - Salivary Proteins and Peptides/analysis
MH  - Secretory Rate
MH  - Statistics as Topic
MH  - Statistics, Nonparametric
MH  - Streptococcus/classification/*immunology
MH  - Streptococcus mutans/immunology
MH  - Streptococcus sobrinus/immunology
EDAT- 2001/02/13 11:00
MHDA- 2002/01/10 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2002/01/10 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - omi160108 [pii]
AID - 10.1034/j.1399-302x.2001.160108.x [doi]
PST - ppublish
SO  - Oral Microbiol Immunol. 2001 Feb;16(1):45-53. doi: 
      10.1034/j.1399-302x.2001.160108.x.