PMID- 11169178
OWN - NLM
STAT- MEDLINE
DCOM- 20010614
LR  - 20240109
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 25
IP  - 1
DP  - 2001 Jan
TI  - A pea homologue of human DNA helicase I is localized within the dense fibrillar 
      component of the nucleolus and stimulated by phosphorylation with CK2 and cdc2 
      protein kinases.
PG  - 9-17
AB  - DNA helicases catalyse the transient opening of duplex DNA during nucleic acid 
      transactions. Here we report the isolation of a second nuclear DNA helicase (65 
      kDa) from Pisum sativum (pea) designated pea DNA helicase 65 (PDH65). The enzyme 
      was immunoaffinity purified using an antihuman DNA helicase I (HDH I) antibody 
      column. The purified PDH65 showed ATP- and Mg(2+)-dependent DNA and RNA unwinding 
      activities, as well as ssDNA-dependent ATPase activity. The direction of DNA 
      unwinding was 3' to 5' along the bound strand. Antibodies against HDH I 
      recognized the purified PDH65, and immunodepletion with these antibodies removed 
      the DNA and RNA unwinding and ATPase activities from purified preparations of 
      PDH65. The DNA and RNA unwinding activities were upregulated after 
      phosphorylation of PDH65 with CK2 and cdc2 protein kinases. By incorporation of 
      BrUTP into pea root tissue, followed by double immunofluorescence labelling and 
      confocal microscopy, PDH65 was shown to be localized within the dense fibrillar 
      component of pea root nucleoli in the regions around the rDNA transcription 
      sites. These observations suggest that PDH65 may be involved both in rDNA 
      transcription and in the early stages of pre-rRNA processing.
FAU - Tuteja, N
AU  - Tuteja N
AD  - International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali 
      Marg, New Delhi 110 070, India. narendra@icgeb.res.in
FAU - Beven, A F
AU  - Beven AF
FAU - Shaw, P J
AU  - Shaw PJ
FAU - Tuteja, R
AU  - Tuteja R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Antibodies)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - EC 2.7.11.22 (CDC2 Protein Kinase)
RN  - EC 3.6.1.- (TraI protein, E coli)
RN  - EC 3.6.4.- (DNA Helicases)
SB  - IM
MH  - Antibodies
MH  - Base Sequence
MH  - CDC2 Protein Kinase/*metabolism
MH  - Cell Nucleolus/*enzymology/ultrastructure
MH  - Chromatography, Affinity
MH  - DNA Helicases/isolation & purification/*metabolism
MH  - Escherichia coli Proteins
MH  - Humans
MH  - Kinetics
MH  - Microscopy, Confocal
MH  - Oligodeoxyribonucleotides/chemistry/metabolism
MH  - Pisum sativum/*enzymology
MH  - Phosphorylation
MH  - Plant Leaves/enzymology
MH  - Substrate Specificity
EDAT- 2001/02/13 11:00
MHDA- 2001/06/23 10:01
CRDT- 2001/02/13 11:00
PHST- 2001/02/13 11:00 [pubmed]
PHST- 2001/06/23 10:01 [medline]
PHST- 2001/02/13 11:00 [entrez]
AID - tpj918 [pii]
AID - 10.1046/j.1365-313x.2001.00918.x [doi]
PST - ppublish
SO  - Plant J. 2001 Jan;25(1):9-17. doi: 10.1046/j.1365-313x.2001.00918.x.