PMID- 11169909
OWN - NLM
STAT- MEDLINE
DCOM- 20010426
LR  - 20231213
IS  - 0003-276X (Print)
IS  - 0003-276X (Linking)
VI  - 262
IP  - 2
DP  - 2001 Feb 1
TI  - Analysis of gap junction formation in rat hepatocytes by intravenous injection of 
      an anti-connexin32 monoclonal antibody (HAM8).
PG  - 147-52
AB  - HAM8 monoclonal antibody was used to examine the mechanism of connexin32 (Cx32) 
      formation in the rat model in vivo by immunofluorescence and immunoelectron 
      microscopy. After a single intravenous injection of HAM8 IgG, a number of HAM8 
      signals were observed at the sinusoidal face, between adjacent hepatocytes as 
      well as in the cytoplasm stained with only 2nd fluorescent antibody. Moreover, 
      the in vivo localization of the HAM8 antigen appeared to change with time. At 5 
      min after the antibody injection, Cx32 signals from liver sections were clearly 
      detected at the sinusoidal face. Fifteen minutes later, numerous linear and 
      dotted fluorescent signals were observed between hepatocytes; in addition, much 
      punctate staining was found at the sinusoidal face and in hepatocytes. These 
      findings were identified by immunoelectron microscopy. Interestingly, 1 hr later, 
      much punctate staining considered to be similar to those seen in the normal rat 
      liver tissues was observed between adjacent hepatocytes, suggesting that a great 
      deal of Cx32 combined with HAM8 have been assembled into identifiable gap 
      junction plaques. Five hours later, intercellular and intracellular Cx32 signals 
      were infrequently detected. When staining was performed with HAM8 and 2nd 
      antibody, however, numerous Cx32 signals were again observed between neighboring 
      hepatocytes, as punctate staining appearing in a pattern approximately the same 
      as that seen in the normal liver tissue. Based on these results, we assumed that 
      a precursor gap was present during Cx32 formation, and discussed the pathways of 
      Cx32 formation and the degradation of Cx32 as well as that of HAM8.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Liu, Z
AU  - Liu Z
AD  - Department of Anatomy (II), Oita Medical University, Idaigaoka, Hasama, Oita, 
      Japan.
FAU - Kitamura, H
AU  - Kitamura H
FAU - Ina, K
AU  - Ina K
FAU - Fukumoto, T
AU  - Fukumoto T
FAU - Fujikura, Y
AU  - Fujikura Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anat Rec
JT  - The Anatomical record
JID - 0370540
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Connexins)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/blood/pharmacology
MH  - Connexins/*biosynthesis/immunology
MH  - Gap Junctions/*metabolism/ultrastructure
MH  - Hepatocytes/*metabolism/ultrastructure
MH  - Immunoglobulin G/blood/pharmacology
MH  - Immunohistochemistry
MH  - Injections, Intravenous
MH  - Male
MH  - Microscopy, Electron
MH  - Microscopy, Fluorescence
MH  - Rats
MH  - Gap Junction beta-1 Protein
EDAT- 2001/02/15 11:00
MHDA- 2001/05/01 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/05/01 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - 10.1002/1097-0185(20010201)262:2<147::AID-AR1020>3.0.CO;2-O [pii]
AID - 10.1002/1097-0185(20010201)262:2<147::AID-AR1020>3.0.CO;2-O [doi]
PST - ppublish
SO  - Anat Rec. 2001 Feb 1;262(2):147-52. doi: 
      10.1002/1097-0185(20010201)262:2<147::AID-AR1020>3.0.CO;2-O.