PMID- 11170182
OWN - NLM
STAT- MEDLINE
DCOM- 20010419
LR  - 20131121
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 63
IP  - 4
DP  - 2001 Feb 15
TI  - Regulation of acetylcholine release by extracellular matrix proteins at 
      developing motoneurons in Xenopus cell cultures.
PG  - 320-9
AB  - Integrins mediate cell-extracellular matrix connections and are particularly 
      important during neuronal development. We here investigated the regulatory role 
      of extracellular matrix (ECM) proteins on the synaptic transmission at developing 
      motoneurons. Synaptic currents were recorded from innervated myocytes of 
      1-day-old Xenopus cultures by whole-cell recordings. Soluble fibronectin and 
      laminin had no significant effect on the frequency of spontaneous synaptic 
      currents (SSCs) by themselves and markedly increased SSC frequency in the 
      presence of low concentration of protein kinase C (PKC) activators. Pretreatment 
      with Gly-Arg-Gly-Asp-Ser peptide inhibited the SSC increasing action of 
      12-o-tetradecanoyl-phorbol-13-acetate (TPA, 0.5 microM) plus fibronectin, but not 
      that of TPA plus laminin. Genistein but not cytochalasin D inhibited the SSC 
      increasing action of TPA plus fibronectin or laminin. High concentration of TPA 
      (5 microM) markedly increased the SSC frequency by itself and occluded the SSC 
      increasing action of fibronectin. Very low concentration of TPA (0.05 microM) 
      markedly enhanced the SSC frequency when the cells were plated onto fibronectin- 
      or laminin-coated substratum for 1 day. The SSC frequency increased markedly 
      right after a train stimulation, which was defined as post-train potentiation 
      (PTrP), when the cultures were plated onto fibronectin substratum and chronically 
      treated with brain-derived neurotrophic factor (BDNF). The PTrP phenomenon is not 
      observed upon chronic treatment with neurotrophin-3, glial cell line-derived 
      neurotrophic factor, or ciliary neurotrophic factor. Our results suggest that the 
      activation of PKC and tyrosine kinase but not actin reorganization plays a role 
      in the SSC potentiating action of fibronectin. BDNF exerts synergistic effects in 
      increasing synaptic transmission in neurons grown on fibronectin substratum. ECMs 
      in concert with neurotrophic factor may play a role in regulating synaptic 
      function at developing motoneurons.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Fu, W M
AU  - Fu WM
AD  - Department of Pharmacology, College of Medicine, National Taiwan University, 
      Taipei, 100, Taiwan. wenmei@ccms.ntu.edu.tw
FAU - Shih, Y C
AU  - Shih YC
FAU - Chen, S Y
AU  - Chen SY
FAU - Tsai, P H
AU  - Tsai PH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carcinogens)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Fibronectins)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Integrins)
RN  - 0 (Laminin)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuroprotective Agents)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - N9YNS0M02X (Acetylcholine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Acetylcholine/*metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Carcinogens/pharmacology
MH  - Cells, Cultured
MH  - Ciliary Neurotrophic Factor/pharmacology
MH  - Extracellular Matrix Proteins/*metabolism
MH  - Fibronectins/pharmacology
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Integrins/metabolism
MH  - Laminin/pharmacology
MH  - Membrane Potentials/drug effects/physiology
MH  - Motor Neurons/cytology/drug effects/*enzymology
MH  - *Nerve Growth Factors
MH  - Nerve Tissue Proteins/pharmacology
MH  - Neuroprotective Agents/pharmacology
MH  - Patch-Clamp Techniques
MH  - Protein Kinase C/*metabolism
MH  - Solubility
MH  - Synaptic Transmission/drug effects/physiology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Xenopus
EDAT- 2001/02/15 11:00
MHDA- 2001/04/21 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/04/21 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - 10.1002/1097-4547(20010215)63:4<320::AID-JNR1026>3.0.CO;2-1 [pii]
AID - 10.1002/1097-4547(20010215)63:4<320::AID-JNR1026>3.0.CO;2-1 [doi]
PST - ppublish
SO  - J Neurosci Res. 2001 Feb 15;63(4):320-9. doi: 
      10.1002/1097-4547(20010215)63:4<320::AID-JNR1026>3.0.CO;2-1.