PMID- 11170284
OWN - NLM
STAT- MEDLINE
DCOM- 20010412
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 30
IP  - 3
DP  - 2001 Mar
TI  - Characterization of complex chromosomal abnormalities in uveal melanoma by 
      fluorescence in situ hybridization, spectral karyotyping, and comparative genomic 
      hybridization.
PG  - 267-73
AB  - Several nonrandom recurrent chromosomal changes are observed in uveal melanoma. 
      Some of these abnormalities, e.g., loss of chromosome 3, gain of the q arm of 
      chromosome 8, and chromosome 6 abnormalities, are of prognostic value. 
      Cytogenetic analysis and/or fluorescence in situ hybridization (FISH) are used to 
      detect these changes. In some cases, however, detailed cytogenetic analysis is 
      not possible due to the presence of complex abnormalities. To define more 
      accurately these cytogenetic changes, we have applied comparative genomic 
      hybridization (CGH) and/or spectral karyotyping (SKY) to two uveal melanoma cell 
      lines and five primary uveal melanomas, with partially defined and/or complex 
      abnormalities. SKY provided additional information on 34/39 partially defined 
      aberrant chromosomes and revealed a new abnormality, a der(17)t(7;17)(?;q?), that 
      had not been recognized by conventional cytogenetics. Additionally, using SKY, 
      abnormalities involving chromosome 6 or 8 were found to be twice as common as 
      observed with cytogenetic analysis. CGH was especially useful in assigning the 
      abnormalities identified by SKY to specific chromosomal regions and, in addition, 
      resulted in the detection of a small deletion of chromosome region 3q13 
      approximately 21. We conclude that SKY and CGH, as methods complementary to 
      cytogenetic and FISH analysis, provide more complete information on the 
      chromosomal abnormalities occurring in uveal melanoma.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Naus, N C
AU  - Naus NC
AD  - Department of Ophthalmology, Erasmus University Rotterdam, Rotterdam, The 
      Netherlands.
FAU - van Drunen, E
AU  - van Drunen E
FAU - de Klein, A
AU  - de Klein A
FAU - Luyten, G P
AU  - Luyten GP
FAU - Paridaens, D A
AU  - Paridaens DA
FAU - Alers, J C
AU  - Alers JC
FAU - Ksander, B R
AU  - Ksander BR
FAU - Beverloo, H B
AU  - Beverloo HB
FAU - Slater, R M
AU  - Slater RM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chromosome Aberrations/*genetics
MH  - Chromosome Disorders
MH  - Chromosomes, Human, Pair 6/genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *Karyotyping/methods
MH  - Male
MH  - Melanoma/*genetics
MH  - Middle Aged
MH  - Nucleic Acid Hybridization
MH  - Tumor Cells, Cultured
MH  - Uveal Neoplasms/*genetics
EDAT- 2001/02/15 11:00
MHDA- 2001/04/17 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/04/17 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - 10.1002/1098-2264(200103)30:3<267::AID-GCC1088>3.0.CO;2-S [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2001 Mar;30(3):267-73.