PMID- 11170791
OWN - NLM
STAT- MEDLINE
DCOM- 20010322
LR  - 20161124
IS  - 0014-4800 (Print)
IS  - 0014-4800 (Linking)
VI  - 70
IP  - 1
DP  - 2001 Feb
TI  - Puromycin aminonucleoside induces glomerular epithelial cell apoptosis.
PG  - 54-64
AB  - Glomerular epithelial cell (GEC) injury has been considered to play an important 
      role in puromycin aminonucleoside (PAN)-induced nephrosis. We studied the effect 
      of PAN on rat as well as human GEC apoptosis. Morphogic evaluation of GEC 
      apoptosis and necrosis was carried out by staining with H-33342 and propidium 
      iodide. GEC apoptosis was further confirmed by DNA fragmentation assay (by both 
      agarose gel electrophoresis and end-labeling). To determine the dose- and 
      time-response effect of PAN, GECs were treated with variable concentrations of 
      PAN (10 to 500 microg/ml) for variable time periods (6 to 48 h). To determine the 
      role of gene synthesis, we studied the effect of actinomycin D (a transcriptional 
      inhibitor) on PAN-induced GEC apoptosis. To determine the role of free radicals, 
      we evaluated the effect of superoxide dismutase (SOD), dimethylthiourea (DMTU), 
      and catalase on PAN-induced GEC apoptosis. PAN induced GEC apoptosis in a dose- 
      and time-dependent manner. PAN at a high concentration (PAN, 100 microg/ml) also 
      induced a moderate degree of GEC necrosis. In DNA fragmentation assays 
      PAN-treated GECs showed the classic ladder pattern. PAN-induced GEC apoptosis was 
      partly attenuated with free radical scavengers, such as SOD, DMTU, and catalase. 
      In addition, actinomycin D attenuated PAN-induced GEC apoptosis. PAN induces GEC 
      apoptosis, which may be mediated through the generation of reactive oxygen 
      species.
FAU - Sanwal, V
AU  - Sanwal V
AD  - Molecular Biology and Experimental Pathology Section, Long Island Jewish Medical 
      Center and North Shore University Hospital, New York, New York 11040, USA.
FAU - Pandya, M
AU  - Pandya M
FAU - Bhaskaran, M
AU  - Bhaskaran M
FAU - Franki, N
AU  - Franki N
FAU - Reddy, K
AU  - Reddy K
FAU - Ding, G
AU  - Ding G
FAU - Kapasi, A
AU  - Kapasi A
FAU - Valderrama, E
AU  - Valderrama E
FAU - Singhal, P C
AU  - Singhal PC
LA  - eng
GR  - R01 DA 12111/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Free Radical Scavengers)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 58-60-6 (Puromycin Aminonucleoside)
RN  - 8P30PMD17W (1,3-dimethylthiourea)
RN  - 9007-49-2 (DNA)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - GYV9AM2QAG (Thiourea)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Catalase/pharmacology
MH  - Cells, Cultured
MH  - DNA/drug effects
MH  - DNA Fragmentation/drug effects
MH  - Dactinomycin/pharmacology
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Electrophoresis, Agar Gel
MH  - Epithelial Cells/drug effects/pathology
MH  - Free Radical Scavengers/pharmacology
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Kidney Glomerulus/*drug effects/pathology
MH  - Puromycin Aminonucleoside/*toxicity
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Superoxide Dismutase/pharmacology
MH  - Thiourea/*analogs & derivatives/pharmacology
EDAT- 2001/02/15 11:00
MHDA- 2001/03/27 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/03/27 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - S0014-4800(00)92345-1 [pii]
AID - 10.1006/exmp.2000.2345 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2001 Feb;70(1):54-64. doi: 10.1006/exmp.2000.2345.