PMID- 11170988
OWN - NLM
STAT- MEDLINE
DCOM- 20010322
LR  - 20061115
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 183
IP  - 4
DP  - 2001 Feb 15
TI  - Expression of Interleukin-18, a Th1 cytokine, in human gastric mucosa is 
      increased in Helicobacter pylori infection.
PG  - 620-7
AB  - Interleukin-18 (IL-18), a cytokine that promotes Th1 responses, is processed to 
      the active mature protein by caspase-1. The effects of Helicobacter pylori 
      infection on gastric IL-18 and caspase-1 were examined. In antral mucosa, IL-18 
      mRNA expression was greater (P<.01) in H. pylori-positive (n=40) than in H. 
      pylori-negative patients (n=29) with normal mucosa. Inactive precursor (24 kDa) 
      and mature (18 kDa) IL-18 were present in antral biopsy specimens from uninfected 
      and infected subjects. In corpus mucosa, mature IL-18 and a 16-kDa protein, 
      corresponding to inactive IL-18, were present. Active caspase-1 p20 subunit was 
      detected in antral and corpus mucosa of infected and uninfected subjects. These 
      data show that, although H. pylori infection is associated with increased antral 
      IL-18 mRNA expression, mature IL-18 protein and active caspase-1 p20 are present 
      in mucosa of both H. pylori-infected and -uninfected subjects. IL-18 may have an 
      important role in promoting gastric Th1 responses in H. pylori infection.
FAU - Tomita, T
AU  - Tomita T
AD  - Molecular Medicine Unit, St. James's University Hospital, Leeds LS9 7TF, United 
      Kingdom.
FAU - Jackson, A M
AU  - Jackson AM
FAU - Hida, N
AU  - Hida N
FAU - Hayat, M
AU  - Hayat M
FAU - Dixon, M F
AU  - Dixon MF
FAU - Shimoyama, T
AU  - Shimoyama T
FAU - Axon, A T
AU  - Axon AT
FAU - Robinson, P A
AU  - Robinson PA
FAU - Crabtree, J E
AU  - Crabtree JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20010124
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Interleukin-18)
RN  - 0 (RNA, Messenger)
RN  - 0 (cagA protein, Helicobacter pylori)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Antigens, Bacterial
MH  - Bacterial Proteins/metabolism
MH  - Caspase 1/metabolism
MH  - Cell Line
MH  - Epithelial Cells
MH  - Female
MH  - Gastric Mucosa/*immunology/metabolism/*microbiology
MH  - Gastritis/diagnosis
MH  - Helicobacter Infections/*immunology/microbiology
MH  - Helicobacter pylori/*immunology
MH  - Humans
MH  - Interleukin-18/*biosynthesis/genetics
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2001/02/15 11:00
MHDA- 2001/03/27 10:01
CRDT- 2001/02/15 11:00
PHST- 2000/09/18 00:00 [received]
PHST- 2000/11/06 00:00 [revised]
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/03/27 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - JID001113 [pii]
AID - 10.1086/318541 [doi]
PST - ppublish
SO  - J Infect Dis. 2001 Feb 15;183(4):620-7. doi: 10.1086/318541. Epub 2001 Jan 24.