PMID- 11170992
OWN - NLM
STAT- MEDLINE
DCOM- 20010322
LR  - 20041117
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 183
IP  - 4
DP  - 2001 Feb 15
TI  - Evidence of porcine endogenous retroviruses in porcine factor VIII and evaluation 
      of transmission to recipients with hemophilia.
PG  - 648-52
AB  - Since 1984, unheated porcine clotting factor VIII (Hyate:C) has been used to 
      treat severe bleeding episodes in persons with hemophilia who have antibodies to 
      human clotting factor. We document the presence of porcine endogenous retrovirus 
      (PERV) in plasma samples of pigs and in clinical lots of Hyate:C. Both gag and 
      pol PERV RNA sequences were detected by reverse-transcriptase (RT) polymerase 
      chain reaction in 13 of 13 lots of Hyate:C tested. Among 10 of these lots, RT 
      activity also was detected, which confirms the presence of retroviral particles. 
      To assess the transmission of PERV to Hyate:C recipients, we tested serum 
      specimens from 88 recipients of Hyate:C and 23 noninfused control subjects for 
      anti-PERV antibodies by using a Western blot assay. None of the samples was 
      positive. Our data document that PERV particles are a common contaminant of 
      Hyate:C products and suggest that the risk of PERV transmission from these 
      percutaneous exposures is very low.
FAU - Heneine, W
AU  - Heneine W
AD  - HIV and Retrovirology Branch, Centers for Disease Control and Prevention, 1600 
      Clifton Road, Atlanta, GA 30333, USA. wmh2@cdc.gov
FAU - Switzer, W M
AU  - Switzer WM
FAU - Soucie, J M
AU  - Soucie JM
FAU - Evatt, B L
AU  - Evatt BL
FAU - Shanmugam, V
AU  - Shanmugam V
FAU - Rosales, G V
AU  - Rosales GV
FAU - Matthews, A
AU  - Matthews A
FAU - Sandstrom, P
AU  - Sandstrom P
FAU - Folks, T M
AU  - Folks TM
LA  - eng
SI  - GENBANK/AF318383
SI  - GENBANK/AF318384
SI  - GENBANK/AF318385
SI  - GENBANK/AF318386
SI  - GENBANK/AF318387
SI  - GENBANK/AF318388
SI  - GENBANK/AF318389
PT  - Journal Article
DEP - 20010112
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Antibodies, Viral)
RN  - 0 (RNA, Viral)
RN  - 9001-27-8 (Factor VIII)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - *Drug Contamination
MH  - Endogenous Retroviruses/classification/genetics/immunology/*isolation & 
      purification
MH  - Factor VIII/*adverse effects
MH  - Hemophilia A/*therapy/virology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Plasma/virology
MH  - RNA, Viral/analysis/blood
MH  - Retroviridae Infections/*transmission/veterinary/virology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Swine/*virology
MH  - Swine Diseases/virology
EDAT- 2001/02/15 11:00
MHDA- 2001/03/27 10:01
CRDT- 2001/02/15 11:00
PHST- 2000/09/14 00:00 [received]
PHST- 2000/11/06 00:00 [revised]
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/03/27 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - JID001108 [pii]
AID - 10.1086/318540 [doi]
PST - ppublish
SO  - J Infect Dis. 2001 Feb 15;183(4):648-52. doi: 10.1086/318540. Epub 2001 Jan 12.