PMID- 11171323
OWN - NLM
STAT- MEDLINE
DCOM- 20010503
LR  - 20220215
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 114
IP  - Pt 3
DP  - 2001 Feb
TI  - Characterization and chromosomal localization of JAM-1, a platelet receptor for a 
      stimulatory monoclonal antibody.
PG  - 539-47
AB  - We have previously reported the purification and characterization of a 32 kDa 
      platelet surface glycoprotein that is recognized by the stimulatory monoclonal 
      antibody, F11. The cDNA has been cloned and found to encode the human homolog of 
      the murine junctional adhesion molecule, JAM; we therefore named this human 
      homolog JAM-1. Northern blot analysis indicated that JAM-1 mRNA is expressed as 
      multiple species, the predominant transcript being approximately 4.0 kb in size. 
      Genetic mapping analysis using fluorescence in situ hybridization (FISH) showed 
      that it is localized to chromosome 1q21.1-21.3. Recombinant JAM-1, when expressed 
      in Chinese hamster ovary (CHO) cells, localized to the cell membrane with intense 
      staining where two adjacent cells actually made contact with each other, 
      suggesting that, similar to murine JAM, human JAM-1 may also localize at the 
      cell-cell junction. In well-spread cells, JAM-1 co-localized with F-actin at the 
      cell-cell contacts and at the membrane ruffles, but not at the stress fibers. 
      Interestingly, JAM-1 localizes only to the cell-cell junctions formed by two 
      transfected cells and not to the cell-cell junctions formed by a transfected cell 
      with an untransfected cell, suggesting that JAM-1 may facilitate cell adhesion 
      through homophilic binding. In addition, human platelets specifically bind to a 
      monolayer of CHO cells expressing human JAM-1, further supporting homophilic 
      interactions. The results presented here indicate that JAM-1, a receptor for a 
      platelet-activating antibody, is the human homolog of the junctional adhesion 
      molecule. JAM-1 is a single copy gene, which is constitutively expressed on 
      various tissues and cells, and may be involved in cell to cell adhesion through 
      homophilic interaction.
FAU - Naik, U P
AU  - Naik UP
AD  - Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA. 
      unaik@udel.edu
FAU - Naik, M U
AU  - Naik MU
FAU - Eckfeld, K
AU  - Eckfeld K
FAU - Martin-DeLeon, P
AU  - Martin-DeLeon P
FAU - Spychala, J
AU  - Spychala J
LA  - eng
GR  - HL57630/HL/NHLBI NIH HHS/United States
GR  - HL63960/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DNA, Complementary)
RN  - 0 (F11R protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Virus)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blood Platelets/cytology
MH  - CHO Cells
MH  - Cell Adhesion
MH  - *Cell Adhesion Molecules
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 1
MH  - Cricetinae
MH  - DNA, Complementary
MH  - Humans
MH  - Membrane Proteins/*genetics
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics/metabolism
MH  - *Receptors, Cell Surface
MH  - Receptors, Virus
MH  - Recombinant Proteins/chemistry/genetics
MH  - Sequence Homology, Amino Acid
MH  - Subcellular Fractions/metabolism
MH  - Tumor Cells, Cultured
EDAT- 2001/02/15 11:00
MHDA- 2001/05/05 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/05/05 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - 10.1242/jcs.114.3.539 [doi]
PST - ppublish
SO  - J Cell Sci. 2001 Feb;114(Pt 3):539-47. doi: 10.1242/jcs.114.3.539.