PMID- 11171393
OWN - NLM
STAT- MEDLINE
DCOM- 20010412
LR  - 20220215
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 128
IP  - 5
DP  - 2001 Mar
TI  - RXR-alpha ablation in skin keratinocytes results in alopecia and epidermal 
      alterations.
PG  - 675-88
AB  - RXR-alpha is the most abundant of the three retinoid X receptors (RXRs) in the 
      epidermis. In this study, we have used Cre-mediated recombination to selectively 
      disrupt the mouse gene for RXR-alpha in epidermal and hair follicle 
      keratinocytes. We show that RXR-alpha is apparently dispensable for prenatal 
      epidermal development, while it is involved in postnatal skin maturation. After 
      the first hair pelage, mutant mice develop a progressive alopecia, histologically 
      characterised by the destruction of hair follicle architecture and the formation 
      of utriculi and dermal cysts in adult mice. Our results demonstrate that 
      RXR-alpha plays a key role in anagen initiation during the hair follicle cycle. 
      In addition, RXR-alpha ablation results in epidermal interfollicular hyperplasia 
      with keratinocyte hyperproliferation and aberrant terminal differentiation, 
      accompanied by an inflammatory reaction of the skin. Our data not only provide 
      genetic evidence that RXR-alpha/VDR heterodimers play a major role in controlling 
      hair cycling, but also suggest that additional signalling pathways mediated by 
      RXR-alpha heterodimerised with other nuclear receptors are involved in postnatal 
      hair follicle growth, and homeostasis of proliferation/differentiation of 
      epidermal keratinocytes and of the skin's immune system.
FAU - Li, M
AU  - Li M
AD  - Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS/INSERM/ULP, 
      College de France, BP 163, 67404 Illkirch Cedex, France.
FAU - Chiba, H
AU  - Chiba H
FAU - Warot, X
AU  - Warot X
FAU - Messaddeq, N
AU  - Messaddeq N
FAU - Gerard, C
AU  - Gerard C
FAU - Chambon, P
AU  - Chambon P
FAU - Metzger, D
AU  - Metzger D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
RN  - 0 (Viral Proteins)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 68238-35-7 (Keratins)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.7.- (Cre recombinase)
RN  - EC 2.7.7.- (Integrases)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Alopecia/genetics/pathology
MH  - Animals
MH  - Blotting, Southern
MH  - Cell Differentiation
MH  - Cloning, Molecular
MH  - Cysts/pathology
MH  - DNA/genetics/metabolism
MH  - Epidermis/*anatomy & histology/growth & development/immunology/ultrastructure
MH  - Female
MH  - Genes, Reporter
MH  - Hair Follicle/growth & development/ultrastructure
MH  - Immunohistochemistry
MH  - Integrases/genetics/metabolism
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Keratinocytes/*cytology/metabolism
MH  - Keratins/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mice, Transgenic
MH  - Plasmids
MH  - Receptors, Retinoic Acid/genetics/*metabolism
MH  - Recombination, Genetic
MH  - Retinoid X Receptors
MH  - T-Lymphocyte Subsets/metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - Transgenes
MH  - *Viral Proteins
MH  - beta-Galactosidase/metabolism
EDAT- 2001/02/15 11:00
MHDA- 2001/04/17 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/04/17 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - 10.1242/dev.128.5.675 [doi]
PST - ppublish
SO  - Development. 2001 Mar;128(5):675-88. doi: 10.1242/dev.128.5.675.