PMID- 11172108
OWN - NLM
STAT- MEDLINE
DCOM- 20010412
LR  - 20231213
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 82
IP  - Pt 3
DP  - 2001 Mar
TI  - Recombination, pseudorecombination and synergism of geminiviruses are determinant 
      keys to the epidemic of severe cassava mosaic disease in Uganda.
PG  - 655-665
LID - 10.1099/0022-1317-82-3-655 [doi]
AB  - The molecular variability of cassava geminiviruses occurring in Uganda was 
      investigated in this study. Infected cassava plants and whiteflies were collected 
      from cassava plantings in different geographical areas of the country and PCR was 
      used for molecular characterization of the viruses. Two complete sequences of 
      DNA-A and -B from African cassava mosaic virus (ACMV), two DNA-A sequences from 
      East African cassava mosaic virus (EACMV), two DNA-B sequences of EACMV and the 
      partial DNA-A nucleotide sequence of a new virus strain isolated in Uganda, 
      EACMV-UG3, are reported here. Analysis of naturally infected cassava plants 
      showed various assortments of DNA-A and DNA-B of the Ugandan viruses, suggesting 
      the occurrence of natural inter- and intraspecies pseudorecombinations and a 
      pattern of cassava mosaic disease (CMD) more complex than previously reported. 
      EACMV-UG2 DNA-A, which contains a recombinant fragment between ACMV and EACMV-UG1 
      in the coat protein gene that resembles virus from Tanzania, was widespread in 
      the country and always associated with EACMV-UG3 DNA-B, which probably resulted 
      from another natural recombination event. Mixed infections of ACMV-UG and 
      EACMV-UG in cassava and whiteflies were detected in most of the regions where 
      both viruses occurred. These mixed-infected samples always showed extremely 
      severe CMD symptoms, suggesting a synergistic interaction between ACMV-UG and 
      EACMV-UG2. The first demonstration is provided of infectivity of EACMV clones to 
      cassava, proving conclusively that the pseudorecombinant EACMV-UG2 
      DNA-A+EACMV-UG3 DNA-B is a causal agent of CMD in Uganda.
FAU - Pita, J S
AU  - Pita JS
AD  - Universite de Cocody, Laboratoire de genetique, 22 BP 582 Abidjan 22, Ivory 
      Coast2.
AD  - International Laboratory for Tropical Agricultural Biotechnology (ILTAB)/Donald 
      Danforth Plant Science Center, UMSL/CME-R308, 8001 Natural Bridge Rd, St Louis, 
      MO 63121-4499, USA1.
FAU - Fondong, V N
AU  - Fondong VN
AD  - Institute of Agronomic Research for Development (IRAD), Ekona PMB 25, Buea South, 
      West Province, Cameroon3.
AD  - International Laboratory for Tropical Agricultural Biotechnology (ILTAB)/Donald 
      Danforth Plant Science Center, UMSL/CME-R308, 8001 Natural Bridge Rd, St Louis, 
      MO 63121-4499, USA1.
FAU - Sangare, A
AU  - Sangare A
AD  - Universite de Cocody, Laboratoire de genetique, 22 BP 582 Abidjan 22, Ivory 
      Coast2.
FAU - Otim-Nape, G W
AU  - Otim-Nape GW
AD  - National Agricultural Research Organization, PO Box 7084, Kampala, Uganda4.
FAU - Ogwal, S
AU  - Ogwal S
AD  - National Agricultural Research Organization, PO Box 7084, Kampala, Uganda4.
FAU - Fauquet, C M
AU  - Fauquet CM
AD  - International Laboratory for Tropical Agricultural Biotechnology (ILTAB)/Donald 
      Danforth Plant Science Center, UMSL/CME-R308, 8001 Natural Bridge Rd, St Louis, 
      MO 63121-4499, USA1.
LA  - eng
SI  - GENBANK/AF126800
SI  - GENBANK/AF126801
SI  - GENBANK/AF126802
SI  - GENBANK/AF126803
SI  - GENBANK/AF126804
SI  - GENBANK/AF126805
SI  - GENBANK/AF126806
SI  - GENBANK/AF126807
SI  - GENBANK/AF230374
SI  - GENBANK/AF230375
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - DNA, Viral/analysis/metabolism
MH  - Geminiviridae/classification/*genetics/physiology
MH  - Genetic Variation
MH  - Hemiptera
MH  - Manihot/*virology
MH  - Molecular Sequence Data
MH  - Plant Diseases/*virology
MH  - Plants, Toxic
MH  - Recombination, Genetic
MH  - Sequence Analysis, DNA/methods
MH  - Nicotiana
MH  - Uganda
EDAT- 2001/02/15 11:00
MHDA- 2001/04/17 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/04/17 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - 10.1099/0022-1317-82-3-655 [doi]
PST - ppublish
SO  - J Gen Virol. 2001 Mar;82(Pt 3):655-665. doi: 10.1099/0022-1317-82-3-655.